Label-Free Single Cell Proteomics Utilizing Ultrafast LC and MS Instrumentation: A Valuable Complementary Technique to Multiplexing.
Sign Up to like & getrecommendations! Published in 2023 at "Proteomics"
DOI: 10.1002/pmic.202200162
Abstract: The ability to map a proteomic fingerprint to transcriptomic data would master the understanding of how gene expression translates into actual phenotype. In contrast to nucleic acid sequencing, in vitro protein amplification is impossible and… read more here.
Keywords: label free; cell; single cell; free single ... See more keywords
Quantitative Accuracy and Precision in Multiplexed Single-Cell Proteomics
Sign Up to like & getrecommendations! Published in 2021 at "Analytical Chemistry"
DOI: 10.1021/acs.analchem.1c04174
Abstract: Single-cell proteomics workflows have considerably improved in sensitivity and reproducibility to characterize as-yet unknown biological phenomena. With the emergence of multiplexed single-cell proteomics, studies increasingly present single-cell measurements in conjunction with an abundant congruent carrier… read more here.
Keywords: multiplexed single; single cell; cell; cell proteomics ... See more keywords
Evaluating Linear Ion Trap for MS3-Based Multiplexed Single-Cell Proteomics.
Sign Up to like & getrecommendations! Published in 2023 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.2c03739
Abstract: There is a growing demand to develop high-throughput and high-sensitivity mass spectrometry methods for single-cell proteomics. The commonly used isobaric labeling-based multiplexed single-cell proteomics approach suffers from distorted protein quantification due to co-isolated interfering ions… read more here.
Keywords: cell; ms3; single cell; ion trap ... See more keywords
Single-Cell Proteomics Using the One-Step Droplet-in-Oil Digestion Method Reveals Proteins Important for Male Meiotic Progression.
Sign Up to like & getrecommendations! Published in 2025 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.4c06467
Abstract: Different from DNA or RNA, proteins cannot be amplified. The performance of single-cell proteomics is limited by sample loss during sample preparation. Here, we present a one-step droplet-in-oil digestion (OSDO) method that involves one-step water-in-oil… read more here.
Keywords: single cell; one step; cell; cell proteomics ... See more keywords
Single Cell Proteomics by Data-Independent Acquisition To Study Embryonic Asymmetry in Xenopus laevis
Sign Up to like & getrecommendations! Published in 2019 at "Analytical Chemistry"
DOI: 10.1021/acs.analchem.9b00327
Abstract: Techniques that allow single cell analysis are gaining widespread attention, and most of these studies utilize genomics-based approaches. While nanofluidic technologies have enabled mass spectromet... read more here.
Keywords: cell proteomics; independent acquisition; proteomics data; data independent ... See more keywords
Protein Adsorption Loss─The Bottleneck of Single-Cell Proteomics.
Sign Up to like & getrecommendations! Published in 2022 at "Journal of proteome research"
DOI: 10.1021/acs.jproteome.2c00317
Abstract: Single-cell proteomics is a promising field to provide direct yet comprehensive molecular insights into cellular functions without averaging effects. Here, we address a grand technical challenge impeding the maturation of single-cell proteomics─protein adsorption loss (PAL).… read more here.
Keywords: cell; protein adsorption; loss; single cell ... See more keywords
Discovery of Oogenesis Biomarkers from Mouse Oocytes Using a Single-Cell Proteomics Approach.
Sign Up to like & getrecommendations! Published in 2023 at "Journal of proteome research"
DOI: 10.1021/acs.jproteome.3c00157
Abstract: We established an efficient and simplified single-cell proteomics (ES-SCP) workflow to realize proteomics profiling at the single-oocyte level. With the ES-SCP workflow, we constructed a deep coverage proteome library during oocyte maturation, which contained more… read more here.
Keywords: discovery oogenesis; protein groups; oocyte maturation; single cell ... See more keywords
Benchmarking In-Cell Proteomics for Profiling Neuroblastoma Cell Differentiation and the Ubiquitin-Proteasome System.
Sign Up to like & getrecommendations! Published in 2025 at "Journal of proteome research"
DOI: 10.1021/acs.jproteome.5c00634
Abstract: The human neuroblastoma SH-SY5Y cell line is a widely utilized model for studying neurodegenerative diseases, owing to its ability to differentiate into cells with a neuron-like phenotype. However, a comprehensive understanding of the cellular and… read more here.
Keywords: cell differentiation; neuroblastoma; cell; cell proteomics ... See more keywords
Single-Cell Proteomics with Spatial Attributes: Tools and Techniques
Sign Up to like & getrecommendations! Published in 2023 at "ACS Omega"
DOI: 10.1021/acsomega.3c00795
Abstract: Now-a-days, the single-cell proteomics (SCP) concept is attracting interest, especially in clinical research, because it can identify the proteomic signature specific to diseased cells. This information is very essential when dealing with the progression of… read more here.
Keywords: spatial attributes; cell; attributes tools; proteomics spatial ... See more keywords
Benchmarking informatics workflows for data-independent acquisition single-cell proteomics
Sign Up to like & getrecommendations! Published in 2025 at "Nature Communications"
DOI: 10.1038/s41467-025-65174-4
Abstract: Recent years have seen a rise of single-cell proteomics by data-independent acquisition mass spectrometry (DIA MS). While diverse data analysis strategies have been reported in literature, their impact on the outcome of single-cell proteomic experiments… read more here.
Keywords: single cell; data independent; analysis; independent acquisition ... See more keywords
Enhanced sensitivity and scalability with a Chip-Tip workflow enables deep single-cell proteomics
Sign Up to like & getrecommendations! Published in 2025 at "Nature Methods"
DOI: 10.1038/s41592-024-02558-2
Abstract: Single-cell proteomics (SCP) promises to revolutionize biomedicine by providing an unparalleled view of the proteome in individual cells. Here, we present a high-sensitivity SCP workflow named Chip-Tip, identifying >5,000 proteins in individual HeLa cells. It… read more here.
Keywords: chip tip; single cell; cell; sensitivity ... See more keywords