Creating Lineage Trajectory Maps Via Integration of Single‐Cell RNA‐Sequencing and Lineage Tracing
Sign Up to like & getrecommendations! Published in 2018 at "BioEssays"
DOI: 10.1002/bies.201800056
Abstract: Mapping the paths that stem and progenitor cells take en route to differentiate and elucidating the underlying molecular controls are key goals in developmental and stem cell biology. However, with population level analyses it is… read more here.
Keywords: single cell; lineage trajectory; lineage; cell rna ... See more keywords
Single cell RNA‐sequencing: A powerful yet still challenging technology to study cellular heterogeneity
Sign Up to like & getrecommendations! Published in 2022 at "BioEssays"
DOI: 10.1002/bies.202200084
Abstract: Almost all biomedical research to date has relied upon mean measurements from cell populations, however it is well established that what it is observed at this macroscopic level can be the result of many interactions… read more here.
Keywords: cell; heterogeneity; rna sequencing; single cell ... See more keywords
Role of stemness-related genes TIMP1, PGF, and SNAI1 in the prognosis of colorectal cancer through single-cell RNA-seq.
Sign Up to like & getrecommendations! Published in 2023 at "Cancer medicine"
DOI: 10.1002/cam4.5833
Abstract: Colorectal cancer (CRC) is a fatal malignant tumor with poor prognosis. Cancer stem cells (CSCs) can cause metastasis, recurrence and drug resistance in CRC. This research aimed to analyze stemness‐related prognostic genes of CRC based… read more here.
Keywords: prognosis; single cell; colorectal cancer; cell rna ... See more keywords
Large-scale single cell RNA sequencing atlases of human immune cells across lifespan: possibilities and challenges.
Sign Up to like & getrecommendations! Published in 2023 at "European journal of immunology"
DOI: 10.1002/eji.202250222
Abstract: Single cell RNA sequencing technologies have been successfully leveraged for immunological insights into human prenatal, paediatric and adult tissues. These single cell studies have led to breakthroughs in our understanding of stem, myeloid and lymphoid… read more here.
Keywords: cell; rna sequencing; large scale; single cell ... See more keywords
In silico analysis of single‐cell RNA sequencing data from 3 and 7 days old mouse spermatogonial stem cells to identify their differentially expressed genes and transcriptional regulators
Sign Up to like & getrecommendations! Published in 2018 at "Journal of Cellular Biochemistry"
DOI: 10.1002/jcb.27066
Abstract: Spermatogonial stem cells (SSCs), which are at the basis of spermatogenesis process, are valuable cells with different applications in biotechnology and regenerative medicine. Understanding the molecular basis of SSC self‐renewal and differentiation at various developmental… read more here.
Keywords: days old; single cell; spermatogonial stem; cell rna ... See more keywords
Single‐cell RNA‐sequencing of retrieved human oocytes and eggs in clinical practice and for human ovarian cell atlasing
Sign Up to like & getrecommendations! Published in 2022 at "Molecular Reproduction and Development"
DOI: 10.1002/mrd.23648
Abstract: With the advancement of single‐cell separation techniques and high‐throughput sequencing platforms, single‐cell RNA‐sequencing (scRNA‐seq) has emerged as a vital technology for understanding tissue and organ systems at cellular resolution. Through transcriptional analysis, it is possible… read more here.
Keywords: cell; rna sequencing; scrna seq; single cell ... See more keywords
Overview of single‐cell RNA sequencing analysis and its application to spermatogenesis research
Sign Up to like & getrecommendations! Published in 2023 at "Reproductive Medicine and Biology"
DOI: 10.1002/rmb2.12502
Abstract: Abstract Background Single‐cell transcriptomics allows parallel analysis of multiple cell types in tissues. Because testes comprise somatic cells and germ cells at various stages of spermatogenesis, single‐cell RNA sequencing is a powerful tool for investigating… read more here.
Keywords: cell; rna sequencing; analysis; single cell ... See more keywords
Single‐Cell RNA Sequencing Reveals the Temporal Diversity and Dynamics of Cardiac Immunity after Myocardial Infarction
Sign Up to like & getrecommendations! Published in 2022 at "Small Methods"
DOI: 10.1002/smtd.202100752
Abstract: Myocardial infarction (MI) is strongly associated with the temporal regulation of cardiac immunity. However, a variety of current clinical trials have failed because of the lack of post‐MI immunomodulating/anti‐inflammatory targets. Single‐cell RNA sequencing analysis of… read more here.
Keywords: cell; cardiac immunity; myocardial infarction; single cell ... See more keywords
Gene Expression Analysis of T-Cells by Single-Cell RNA-Seq.
Sign Up to like & getrecommendations! Published in 2021 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-1311-5_22
Abstract: During the last decade, the rapid progress in the development of next-generation sequencing (NGS) technologies has provided relevant insights into complex biological systems, ranging from cancer genomics to microbiology. Among NGS technologies, single-cell RNA sequencing… read more here.
Keywords: single cell; gene expression; cell rna; expression analysis ... See more keywords
Methods for Characterization of Senescent Circulating and Tumor-Infiltrating T-Cells: An Overview from Multicolor Flow Cytometry to Single-Cell RNA Sequencing.
Sign Up to like & getrecommendations! Published in 2021 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-1507-2_6
Abstract: Immunosenescence is the general term used to describe the aging-associated decline of immunological function that explains the higher susceptibility to infectious diseases and cancer, increased autoimmunity, or the reduced effectiveness of vaccinations. Senescence of CD8+… read more here.
Keywords: single cell; multicolor flow; cell rna; rna sequencing ... See more keywords
A Single-Cell RNA-Seq-Based Approach for Genome-Wide Identification of Cell Essential Genes.
Sign Up to like & getrecommendations! Published in 2022 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-1720-5_18
Abstract: Identification of genes essential for structure, function, and survival of a cell type is critical for understanding of the underlying mechanisms. Unfortunately, there is no efficient way to identify such genes. Studies by single-cell RNA… read more here.
Keywords: single cell; cell rna; essential genes; approach ... See more keywords