Single-Cell Single-Molecule Pull-Down (sc-SiMPull) for Detection of Protein Complexes from Embryonic Lysates.
Sign Up to like & getrecommendations! Published in 2022 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-2035-9_4
Abstract: Mapping how proteins form complexes and change binding partners is central to understanding cell signaling. Bulk biochemistry can provide a summary of what complexes are present in a cell, but information about the diversity of… read more here.
Keywords: protein complexes; single cell; cell; molecule pull ... See more keywords
Live-Cell Single RNA Imaging Reveals Bursts of Translational Frameshifting.
Sign Up to like & getrecommendations! Published in 2019 at "Molecular cell"
DOI: 10.1016/j.molcel.2019.05.002
Abstract: Ribosomal frameshifting during the translation of RNA is implicated in human disease and viral infection. While previous work has uncovered many details about single RNA frameshifting kinetics in vitro, little is known about how single RNA… read more here.
Keywords: live cell; rna; cell single; single rna ... See more keywords
Live cell single molecule-guided Bayesian localization super resolution microscopy
Sign Up to like & getrecommendations! Published in 2017 at "Cell Research"
DOI: 10.1038/cr.2016.160
Abstract: Cell culture, transfection and fixation U2OS cells in McCoy's 5A Medium Modified (MCMM) (Life Technologies), HeLa cells and COS7 cells in Dulbecco’s Modified Eagle Medium (DMEM) supplemented with glucose (Life Technologies) and 10% fetal bovine… read more here.
Keywords: live cell; microscopy; cell single; life technologies ... See more keywords
Cumulus provides cloud-based data analysis for large-scale single-cell and single-nucleus RNA-seq
Sign Up to like & getrecommendations! Published in 2020 at "Nature methods"
DOI: 10.1038/s41592-020-0905-x
Abstract: Massively parallel single-cell and single-nucleus RNA sequencing has opened the way to systematic tissue atlases in health and disease, but as the scale of data generation is growing, so is the need for computational pipelines… read more here.
Keywords: single cell; large scale; single nucleus; cell single ... See more keywords
Live-cell single-molecule labeling and analysis of myosin motors with quantum dots
Sign Up to like & getrecommendations! Published in 2017 at "Molecular Biology of the Cell"
DOI: 10.1091/mbc.e16-06-0413
Abstract: Quantum dots (QDs) are a powerful tool for quantitative biology, but two challenges are associated with using them to track intracellular molecules in live cells. A simple and convenient method is presented for labeling intracellular… read more here.
Keywords: single molecule; live cell; cell single; biology ... See more keywords
Bistable Josephson cell as a single microwave photon sensor
Sign Up to like & getrecommendations! Published in 2020 at "International Journal of Quantum Information"
DOI: 10.1142/s0219749919410144
Abstract: The dissipative dynamics of a bistable Josephson cell in a transmission line resonator in a weakly dissipative regime was studied theoretically and numerically. The bistable cell consists of a quan... read more here.
Keywords: single microwave; cell single; bistable josephson; cell ... See more keywords
Single-cell and single-nuclei RNA sequencing as powerful tools to decipher cellular heterogeneity and dysregulation in neurodegenerative diseases
Sign Up to like & getrecommendations! Published in 2022 at "Frontiers in Cell and Developmental Biology"
DOI: 10.3389/fcell.2022.884748
Abstract: Neurodegenerative diseases affect millions of people worldwide and there are currently no cures. Two types of common neurodegenerative diseases are Alzheimer’s (AD) and Parkinson’s disease (PD). Single-cell and single-nuclei RNA sequencing (scRNA-seq and snRNA-seq) have… read more here.
Keywords: cell; cell single; single cell; nuclei rna ... See more keywords