Comparison of electrostatic and mechanical cell sorting with limited starting material
Sign Up to like & getrecommendations! Published in 2021 at "Cytometry Part A"
DOI: 10.1002/cyto.a.24523
Abstract: Isolation of multiple cell populations from limited starting material and with minimal influence on cell homeostasis and viability are common requirements in both basic and clinical research. Fluorescence‐activated cell sorting (FACS) is the most commonly… read more here.
Keywords: cell sorting; viability; limited starting; starting material ... See more keywords
Establishment of a pure culture of immature Sertoli cells by PDGFRA staining and cell sorting
Sign Up to like & getrecommendations! Published in 2022 at "Molecular Reproduction and Development"
DOI: 10.1002/mrd.23574
Abstract: Sertoli cells are key somatic cells in the testis that form seminiferous tubules and support spermatogenesis. The isolation of pure Sertoli cells is important for their study. However, it is a difficult effort because of… read more here.
Keywords: sertoli; cell; establishment pure; cell sorting ... See more keywords
Multiparameter Fluorescence-Activated Cell Sorting of Human Lymphatic Endothelial Cells.
Sign Up to like & getrecommendations! Published in 2022 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-2217-9_3
Abstract: Multiparameter fluorescence-activated cell sorting (FACS) procedure separates target cells from a total population of cells by using specific signatures that the target cell expresses on their cell surface. For human lymphatic endothelial cells (LECs) this… read more here.
Keywords: activated cell; fluorescence activated; cell sorting; cell ... See more keywords
Fluorescence-Activated Cell Sorting of Murine Mammary Cancer Stem-Like Cell Subpopulations with HIF Activity.
Sign Up to like & getrecommendations! Published in 2018 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-7665-2_22
Abstract: Fluorescence-activated cell sorting (FACS) is a common method to identify and to isolate subpopulations within a complex mixture of cells based on their light scatter and fluorescent staining profiles. FACS is widely used to enrich… read more here.
Keywords: activated cell; cell sorting; fluorescence activated; cancer ... See more keywords
Isolating Embryonic Cardiac Progenitors and Cardiac Myocytes by Fluorescence-Activated Cell Sorting.
Sign Up to like & getrecommendations! Published in 2018 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-7714-7_9
Abstract: Isolation of highly purified populations of embryonic cardiomyocytes enables the study of congenital cardiac phenotypes at the cellular level. Fluorescent-activated cell sorting (FACS) is normally used to isolate fluorescently tagged cells. Here we describe the… read more here.
Keywords: cardiac progenitors; activated cell; isolating embryonic; cell sorting ... See more keywords
Identifying Differentially Expressed Genes Using Fluorescence-Activated Cell Sorting (FACS) and RNA Sequencing from Low Input Samples.
Sign Up to like & getrecommendations! Published in 2018 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-8618-7_6
Abstract: Cell type-specific gene expression profiles are useful for understanding genes that are important for the development of different tissues and organs. Here, we describe how to perform fluorescence-activated cell sorting (FACS) on Arabidopsis root protoplasts… read more here.
Keywords: activated cell; cell sorting; sorting facs; rna sequencing ... See more keywords
Isolation of Anti-Hapten Antibodies by Fluorescence-Activated Cell Sorting of Yeast-Displayed B-Cell Receptor Gene Repertoires.
Sign Up to like & getrecommendations! Published in 2020 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-9853-1_15
Abstract: Anti-hapten antibodies are widely used as specific immunochemical detection tools in a variety of clinical and environmental analyses. The sensitivity, however, is limited due to the resulting antibody affinities to the haptens which, in turn,… read more here.
Keywords: activated cell; hapten antibodies; cell sorting; fluorescence activated ... See more keywords
Development overview of Raman-activated cell sorting devoted to bacterial detection at single-cell level.
Sign Up to like & getrecommendations! Published in 2021 at "Applied microbiology and biotechnology"
DOI: 10.1007/s00253-020-11081-1
Abstract: Understanding the metabolic interactions between bacteria in natural habitat at the single-cell level and the contribution of individual cell to their functions is essential for exploring the dark matter of uncultured bacteria. The combination of… read more here.
Keywords: single cell; activated cell; raman activated; cell sorting ... See more keywords
Inertial Microfluidics-Based Cell Sorting
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DOI: 10.1007/s13206-018-2401-2
Abstract: Inertial microfluidics has attracted significant attention in recent years due to its superior benefits of high throughput, precise control, simplicity, and low cost. Many inertial microfluidic applications have been demonstrated for physiological sample processing, clinical… read more here.
Keywords: microfluidics based; cell sorting; inertial microfluidics; based cell ... See more keywords
Intelligent Image-Activated Cell Sorting
Sign Up to like & getrecommendations! Published in 2018 at "Cell"
DOI: 10.1016/j.cell.2018.08.028
Abstract: A fundamental challenge of biology is to understand the vast heterogeneity of cells, particularly how cellular composition, structure, and morphology are linked to cellular physiology. Unfortunately, conventional technologies are limited in uncovering these relations. We… read more here.
Keywords: activated cell; cell sorting; cell; image activated ... See more keywords
Efficient recovery of potent tumour-infiltrating lymphocytes through quantitative immunomagnetic cell sorting.
Sign Up to like & getrecommendations! Published in 2022 at "Nature biomedical engineering"
DOI: 10.1038/s41551-021-00820-y
Abstract: Adoptive cell therapies require the recovery and expansion of highly potent tumour-infiltrating lymphocytes (TILs). However, TILs in tumours are rare and difficult to isolate efficiently, which hinders the optimization of therapeutic potency and dose. Here… read more here.
Keywords: cell sorting; immunomagnetic cell; tumour infiltrating; potent tumour ... See more keywords