Live-Cell STED Imaging with the HyPer2 Biosensor.
Sign Up to like & getrecommendations! Published in 2017 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-7265-4_3
Abstract: Stimulated emission depletion (STED) microscopy is a popular super resolution imaging technique. Not only synthetic dyes and fluorescent proteins can be utilized as STED fluorophores, but also genetically encoded biosensors. Fusing the biosensor with proteins… read more here.
Keywords: hyper2 biosensor; biosensor; cell sted; live cell ... See more keywords
New coumarin- and phenoxazine-based fluorescent probes for live-cell STED nanoscopy
Sign Up to like & getrecommendations! Published in 2019 at "European Biophysics Journal"
DOI: 10.1007/s00249-019-01354-7
Abstract: The potential of live-cell stimulated emission depletion (STED) nanoscopy has not yet been fully exploited. Currently, the main limitation is the small number of fluorophores and probes that can sustain high light intensity/high dose employed… read more here.
Keywords: live cell; tested probes; cell sted; sted nanoscopy ... See more keywords
Live-cell STED nanoscopy of mitochondrial cristae
Sign Up to like & getrecommendations! Published in 2019 at "Scientific Reports"
DOI: 10.1038/s41598-019-48838-2
Abstract: Mitochondria are highly dynamic organelles that exhibit a complex inner architecture. They exhibit a smooth outer membrane and a highly convoluted inner membrane that forms invaginations called cristae. Imaging cristae in living cells poses a… read more here.
Keywords: live cell; cristae; nanoscopy mitochondrial; cell sted ... See more keywords
Clearing of hemozoin crystals in malaria parasites enables whole cell STED microscopy.
Sign Up to like & getrecommendations! Published in 2022 at "Journal of cell science"
DOI: 10.1242/jcs.260399
Abstract: Malaria is a devastating mosquito-borne parasitic disease that manifests when Plasmodium parasites replicate within red blood cells. During the development within the red cell, the parasite digests hemoglobin and crystalizes the otherwise toxic heme. The… read more here.
Keywords: cell; microscopy; clearing hemozoin; whole cell ... See more keywords