Implementing microwell slides for detection and isolation of single circulating tumor cells from complex cell suspensions
Sign Up to like & getrecommendations! Published in 2022 at "Cytometry Part A"
DOI: 10.1002/cyto.a.24660
Abstract: Cell loss during detection and isolation of circulating tumor cells (CTCs) is a challenge especially when label‐free pre‐enrichment technologies are used without the aid of magnetic particles. Although microfluidic systems can remove the majority of… read more here.
Keywords: cell suspensions; cell; complex cell; detection ... See more keywords
A novel filtration system for point of care washing of cellular therapy products
Sign Up to like & getrecommendations! Published in 2017 at "Journal of Tissue Engineering and Regenerative Medicine"
DOI: 10.1002/term.2225
Abstract: The cell therapy industry would greatly benefit from a simple point of care solution to remove dimethylsulphoxide (DMSO) from small‐volume thawed cell suspensions before injection. A novel dead‐end filtration device has been designed and validated,… read more here.
Keywords: point care; filtration; cell suspensions; novel filtration ... See more keywords
A rational approach for the improvement of biomass production and lipid profile in cacao cell suspensions
Sign Up to like & getrecommendations! Published in 2017 at "Bioprocess and Biosystems Engineering"
DOI: 10.1007/s00449-017-1805-z
Abstract: Cocoa butter (CB) is produced in the seeds of Theobroma cacao representing 50% of its dry weight. The lipid composition plays an important role in the physicochemical, rheological, and sensory properties of the CB, making… read more here.
Keywords: lipid profile; production; biomass production; cell suspensions ... See more keywords
Comparative analysis of cyclotide-producing plant cell suspensions presents opportunities for cyclotide plant molecular farming.
Sign Up to like & getrecommendations! Published in 2021 at "Phytochemistry"
DOI: 10.1016/j.phytochem.2021.113053
Abstract: Cyclotides are a class of ribosomally-synthesized plant peptides that function in plants as a defense against insects and fungal pathogens. Their unique structure comprises a cyclized peptide backbone threaded by three disulfide bonds, that imparts… read more here.
Keywords: expression; cell suspensions; cyclotide; plant ... See more keywords
Critical Functionality Effects from Storage Temperature on Human Induced Pluripotent Stem Cell-Derived Retinal Pigment Epithelium Cell Suspensions
Sign Up to like & getrecommendations! Published in 2018 at "Scientific Reports"
DOI: 10.1038/s41598-018-38065-6
Abstract: Human induced pluripotent stem cell (hiPSC)-derived retinal pigment epithelium (hiPSC-RPE) cells suspension have the potential for regenerative treatment. However, practical regenerative applications with hiPSC-RPE cells require the development of simple and cost-effective non-freezing preservation methods.… read more here.
Keywords: temperature; human induced; cell suspensions; hipsc rpe ... See more keywords
Preparation of Intact Nuclei for Single-Nucleus Omics Using Frozen Cell Suspensions from Mutant Embryos of Xenopus tropicalis.
Sign Up to like & getrecommendations! Published in 2022 at "Cold Spring Harbor protocols"
DOI: 10.1101/pdb.prot107825
Abstract: Single-cell omics such as single-cell RNA-sequencing (RNA-seq) have been used extensively to obtain single-cell genome-wide expression data. This technique can be used to compare mutant and wild-type embryos at predifferentiation stages when individual tissues are… read more here.
Keywords: cell suspensions; cell; seq; single nucleus ... See more keywords
A Method for Collecting Single Cell Suspensions Using an Ultrasonic Pump
Sign Up to like & getrecommendations! Published in 2018 at "IEEE Transactions on Biomedical Engineering"
DOI: 10.1109/tbme.2017.2699291
Abstract: The presence of cell aggregates in cell suspensions may reduce cell culture efficiency because they can induce apoptosis and inhibit proliferation. To avoid this problem, this study proposes a novel method for collecting single cell… read more here.
Keywords: cell culture; method; cell suspensions; culture ... See more keywords
A double-well potential model for the ionic electrokinetic polarization in low concentration cell suspensions
Sign Up to like & getrecommendations! Published in 2019 at "IEEE Transactions on Dielectrics and Electrical Insulation"
DOI: 10.1109/tdei.2019.007980
Abstract: In order to understand the effects of cells on the ionic electrokinetic polarization process in cell suspensions, a double-well potential electrokinetic polarization model for ions in cell suspensions under a pressure gradient field is established… read more here.
Keywords: ionic electrokinetic; cell suspensions; electrokinetic polarization; polarization ... See more keywords
Analysis of Volatile Secondary Metabolites in Ocimum basilicum Cell Suspensions: Inhibition, In Silico Molecular Docking, and an ADMET Analysis against Proteolytic Enzymes of Rhynchophorus ferrugineus
Sign Up to like & getrecommendations! Published in 2022 at "Plants"
DOI: 10.3390/plants11212949
Abstract: Our study’s overarching goal was to determine which O. basilicum cell suspensions approach yielded the most insecticidal and R. ferrugineus-inhibitory volatile secondary metabolites. After inoculation with Verticillium dahliae as an activator, the growth kinetics were… read more here.
Keywords: cell suspensions; secondary metabolites; analysis; basilicum ... See more keywords