Enhanced cellobiose fermentation by engineered Saccharomyces cerevisiae expressing a mutant cellodextrin facilitator and cellobiose phosphorylase.
Sign Up to like & getrecommendations! Published in 2018 at "Journal of biotechnology"
DOI: 10.1016/j.jbiotec.2018.04.008
Abstract: To efficiently ferment intermediate cellodextrins released during cellulose hydrolysis, Saccharomyces cerevisiae has been engineered by introduction of a heterologous cellodextrin utilizing pathway consisting of a cellodextrin transporter and either an intracellular β-glucosidase or a cellobiose… read more here.
Keywords: cellobiose fermentation; cellodextrin; cdt; cellobiose phosphorylase ... See more keywords
Relief of Xylose Binding to Cellobiose Phosphorylase by a Single Distal Mutation.
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DOI: 10.1021/acssynbio.6b00211
Abstract: Cellobiose phosphorylase (CBP) cleaves cellobiose-abundant in plant biomass-to glucose and glucose 1-phosphate. However, the pentose sugar xylose, also abundant in plant biomass, acts as a mixed-inhibitor and a substrate for the reverse reaction, limiting the… read more here.
Keywords: distal mutation; relief xylose; cellobiose phosphorylase; xylose binding ... See more keywords