Measuring calibration factors by imaging a dish of cells expressing different tandem constructs plasmids
Sign Up to like & getrecommendations! Published in 2021 at "Cytometry Part A"
DOI: 10.1002/cyto.a.24316
Abstract: Three‐cube Förster resonance energy transfer (FRET) method is the most extensively applied approach for live‐cell FRET quantification. Reliable measurements of calibration factors are crucial for quantitative FRET measurement. We here proposed a modified TA‐G method… read more here.
Keywords: expressing different; cells expressing; method; calibration factors ... See more keywords
RUNX‐mediated growth arrest and senescence are attenuated by diverse mechanisms in cells expressing RUNX1 fusion oncoproteins
Sign Up to like & getrecommendations! Published in 2018 at "Journal of Cellular Biochemistry"
DOI: 10.1002/jcb.26443
Abstract: RUNX gene over‐expression inhibits growth of primary cells but transforms cells with tumor suppressor defects, consistent with reported associations with tumor progression. In contrast, chromosomal translocations involving RUNX1 are detectable in utero, suggesting an initiating… read more here.
Keywords: expressing runx1; cells expressing; senescence; runx1 fusion ... See more keywords
TRPM2 modulates neutrophil attraction to murine tumor cells by regulating CXCL2 expression
Sign Up to like & getrecommendations! Published in 2018 at "Cancer Immunology, Immunotherapy"
DOI: 10.1007/s00262-018-2249-2
Abstract: In recent years, immune cells were shown to play critical roles in tumor growth and metastatic progression. In this context, neutrophils were shown to possess both pro- and anti-tumor properties. To exert their anti-tumor effect,… read more here.
Keywords: trpm2 modulates; cells expressing; tumor cells; levels trpm2 ... See more keywords
Fab-conjugated PLGA nanoparticles effectively target cancer cells expressing human CD44v6.
Sign Up to like & getrecommendations! Published in 2018 at "Acta biomaterialia"
DOI: 10.1016/j.actbio.2018.09.043
Abstract: Targeting of CD44 isoforms containing exon v6 (CD44v6) represents a viable strategy for the therapy and/or early diagnosis of metastatic cancers of the epithelium (e.g. gastric and colorectal cancer). We developed and characterized poly(lactic-co-glycolic acid)… read more here.
Keywords: cells expressing; cd44v6; fab plga; plga nps ... See more keywords
Kinetic profiles of photocurrents in cells expressing two types of channelrhodopsin genes.
Sign Up to like & getrecommendations! Published in 2018 at "Biochemical and biophysical research communications"
DOI: 10.1016/j.bbrc.2018.01.149
Abstract: Channelrhodopsin-2 (ChR2), a light-activated cation-selective ion channel, has been widely used as a tool in optogenetic research. ChR2 is specifically sensitive to wavelengths less than 550 nm. One of the methods to expand the sensitivity of… read more here.
Keywords: expressing two; cells expressing; kinetic profiles; channelrhodopsin ... See more keywords
Novel Approach to Study SERCA Function In Situ
Sign Up to like & getrecommendations! Published in 2017 at "Biophysical Journal"
DOI: 10.1016/j.bpj.2016.11.2165
Abstract: Previously used techniques to measure the sarcoplasmic/endoplasmic reticulum Ca-ATPase (SERCA) function either are cell destructive or lack sensitivity. We designed a new approach that allows studying SERCA function in the cellular environment under well-controlled conditions.… read more here.
Keywords: serca function; cells expressing; study; serca2a ... See more keywords
Engineered regulatory T cells expressing myelin-specific chimeric antigen receptors suppress EAE progression.
Sign Up to like & getrecommendations! Published in 2020 at "Cellular immunology"
DOI: 10.1016/j.cellimm.2020.104222
Abstract: The expansion of polyclonal T regulatory cells (Tregs) offers great promise for the treatment of immune-mediated diseases, such as multiple sclerosis (MS). However, polyclonal Tregs can be non-specifically immunosuppressive. Based on the advancements with chimeric… read more here.
Keywords: cells expressing; regulatory cells; antigen receptors; car ... See more keywords
Single cell expression analysis of primate-specific retroviruses-derived HPAT lincRNAs in viable human blastocysts identifies embryonic cells co-expressing genetic markers of multiple lineages
Sign Up to like & getrecommendations! Published in 2018 at "Heliyon"
DOI: 10.1016/j.heliyon.2018.e00667
Abstract: Chromosome instability and aneuploidies occur very frequently in human embryos, impairing proper embryogenesis and leading to cell cycle arrest, loss of cell viability, and developmental failures in 50–80% of cleavage-stage embryos. This high frequency of… read more here.
Keywords: single cell; embryonic cells; cells expressing; expression ... See more keywords
A single amino acid polymorphism in ABCC2 loop 1 is responsible for differential toxicity of Bacillus thuringiensis Cry1Ac toxin in different Spodoptera (Noctuidae) species.
Sign Up to like & getrecommendations! Published in 2018 at "Insect biochemistry and molecular biology"
DOI: 10.1016/j.ibmb.2018.06.004
Abstract: Bacillus thuringiensis Cry toxins exert their toxicity by forming membrane pores after binding with larval midgut membrane proteins known as receptors. Spodoptera litura and Spodoptera frugiperda belong to the same genus, but S. litura is… read more here.
Keywords: amino acid; cells expressing; cry1ac; differential toxicity ... See more keywords
Classic and HIV‐related Kaposi sarcoma treated with 0.1% topical timolol gel
Sign Up to like & getrecommendations! Published in 2017 at "Journal of the American Academy of Dermatology"
DOI: 10.1016/j.jaad.2016.08.041
Abstract: T cells, or CD56 NK cells expressing NKG2D. We collected blood samples 4 times, and the FACS analysis was also performed by dividing into 4 times. Therefore, final data were represented as a fold change… read more here.
Keywords: classic hiv; nkg2dcd4 cells; expressing nkg2d; cells expressing ... See more keywords
Efficient and long-term vanillin production from 4-vinylguaiacol using immobilized whole cells expressing Cso2 protein.
Sign Up to like & getrecommendations! Published in 2020 at "Journal of bioscience and bioengineering"
DOI: 10.1016/j.jbiosc.2020.04.012
Abstract: Vanillin is a well-known fragrant, flavoring compound. Previously, we established a method of coenzyme-independent vanillin production via an oxygenase from Caulobacter segnis ATCC 21756, called Cso2, that converts 4-vinylguaiacol to vanillin and formaldehyde using oxygen.… read more here.
Keywords: vanillin production; expressing cso2; vanillin; cells expressing ... See more keywords