Expansion and Determination of Antigen-Reactive T Cells by Flow Cytometry.
Sign Up to like & getrecommendations! Published in 2019 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-8979-9_10
Abstract: The detection of antigen-reactive T cells has shown great utility for patient clinical monitoring. However, many of the methods commonly used to detect these cells face major limitations, like the predetermination of the given HLA… read more here.
Keywords: determination antigen; expansion determination; reactive cells; antigen reactive ... See more keywords
Cells´ Flow and Immune Cell Priming under alternating g-forces in Parabolic Flight
Sign Up to like & getrecommendations! Published in 2019 at "Scientific Reports"
DOI: 10.1038/s41598-019-47655-x
Abstract: Gravitational stress in general and microgravity (µg) in particular are regarded as major stress factors responsible for immune system dysfunction in space. To assess the effects of alternating µg and hypergravity (hyper-g) on immune cells,… read more here.
Keywords: immune; immune cell; parabolic flight; cells flow ... See more keywords
T cells go with the flow: Aquaporin 4 is required for full T cell activation.
Sign Up to like & getrecommendations! Published in 2023 at "Journal of leukocyte biology"
DOI: 10.1093/jleuko/qiad041
Abstract: Proximal T-cell receptor signaling and subsequent T cell activation was reduced downstream of anti-CD3/CD28 ligation through small molecule inhibition of the water channel aquaporin 4. read more here.
Keywords: flow aquaporin; cell; aquaporin required; cell activation ... See more keywords
Effects of shear stress on endothelial cells: go with the flow
Sign Up to like & getrecommendations! Published in 2017 at "Acta Physiologica"
DOI: 10.1111/apha.12725
Abstract: Haemodynamic forces influence the functional properties of vascular endothelium. Endothelial cells (ECs) have a variety of receptors, which sense flow and transmit mechanical signals through mechanosensitive signalling pathways to recipient molecules that lead to phenotypic… read more here.
Keywords: effects shear; regions exposed; shear stress; endothelial cells ... See more keywords
Simple In-liquid Staining of Microbial Cells for Flow Cytometry Quantification of the Microbial Population in Marine Subseafloor Sediments
Sign Up to like & getrecommendations! Published in 2021 at "Microbes and Environments"
DOI: 10.1264/jsme2.me21031
Abstract: Microbial cell counting provides essential information for the study of cell abundance profiles and biogeochemical interactions with the surrounding environments. However, it often requires labor-intensive and time-consuming processes, particularly for subseafloor sediment samples, in which… read more here.
Keywords: microscopy; cell; cells flow; staining microbial ... See more keywords