Analysis of Cellular DNA Content by Flow Cytometry
Sign Up to like & getrecommendations! Published in 2017 at "Current Protocols in Immunology"
DOI: 10.1002/cpim.36
Abstract: Cellular DNA content can be measured by flow cytometry with the aim of : (1) revealing cell distribution within the major phases of the cell cycle, (2) estimating frequency of apoptotic cells with fractional DNA… read more here.
Keywords: flow cytometry; cellular dna; dna content; dna ... See more keywords
Three-Component Bioorthogonal Reactions on Cellular DNA and RNA.
Sign Up to like & getrecommendations! Published in 2019 at "Bioconjugate chemistry"
DOI: 10.1021/acs.bioconjchem.9b00630
Abstract: Metabolic incorporation of bioorthogonal functional groups into chromatin, followed by copper-free conjugation reactions, often gives low yields due to steric hindrance. Here we report that a cationic Sondheimer diyne derivative "DiMOC" rapidly reacts with azide… read more here.
Keywords: cellular dna; dimoc; dna; component bioorthogonal ... See more keywords
Reconsidering the Chemical Nature of Strand Breaks Derived from Abasic Sites in Cellular DNA: Evidence for 3'-Glutathionylation.
Sign Up to like & getrecommendations! Published in 2022 at "Journal of the American Chemical Society"
DOI: 10.1021/jacs.2c02703
Abstract: The hydrolytic loss of coding bases from cellular DNA is a common and unavoidable reaction. The resulting abasic sites can undergo β-elimination of the 3'-phosphoryl group to generate a strand break with an electrophilic α,β-unsaturated… read more here.
Keywords: evidence; cellular dna; strand; strand break ... See more keywords
Review of the Geant4-DNA Simulation Toolkit for Radiobiological Applications at the Cellular and DNA Level
Sign Up to like & getrecommendations! Published in 2021 at "Cancers"
DOI: 10.3390/cancers14010035
Abstract: Simple Summary A brief description of the methodologies to simulate ionizing radiation transport in biologically relevant matter is presented. Emphasis is given to the physical, chemical, and biological models of Geant4-DNA that enable mechanistic radiobiological… read more here.
Keywords: dna; dna level; simulation; cellular dna ... See more keywords
Parvovirus minute virus of mice interacts with sites of cellular DNA damage to establish and amplify its lytic infection
Sign Up to like & getrecommendations! Published in 2018 at "eLife"
DOI: 10.7554/elife.37750
Abstract: We have developed a generally adaptable, novel high-throughput Viral Chromosome Conformation Capture assay (V3C-seq) for use in trans that allows genome-wide identification of the direct interactions of a lytic virus genome with distinct regions of… read more here.
Keywords: dna damage; cellular dna; infection; damage ... See more keywords