High-Throughput Cellular Thermal Shift Assay Using Acoustic Transfer of Protein Lysates.
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DOI: 10.1021/acschembio.1c00760
Abstract: Cellular thermal shift assay (CETSA) is a valuable method to confirm target engagement within a complex cellular environment, by detecting changes in a protein's thermal stability upon ligand binding. The classical CETSA method measures changes… read more here.
Keywords: using acoustic; thermal shift; shift assay; high throughput ... See more keywords
Real-Time Cellular Thermal Shift Assay to Monitor Target Engagement
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DOI: 10.1021/acschembio.2c00334
Abstract: Determining a molecule’s mechanism of action is paramount during chemical probe development and drug discovery. The cellular thermal shift assay (CETSA) is a valuable tool to confirm target engagement in cells for a small molecule… read more here.
Keywords: cellular thermal; real time; target engagement; cetsa ... See more keywords
Determining direct binders of the Androgen Receptor using a high-throughput Cellular Thermal Shift Assay
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DOI: 10.1038/s41598-017-18650-x
Abstract: Androgen Receptor (AR) is a key driver in prostate cancer. Direct targeting of AR has valuable therapeutic potential. However, the lack of disease relevant cellular methodologies capable of discriminating between inhibitors that directly bind AR… read more here.
Keywords: direct binders; shift assay; cellular thermal; receptor ... See more keywords
Proteome-wide cellular thermal shift assay reveals unexpected cross-talk between brassinosteroid and auxin signaling
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DOI: 10.1073/pnas.2118220119
Abstract: Significance Chemical genetics, which investigates biological processes using small molecules, is gaining interest in plant research. However, a major challenge is to uncover the mode of action of the small molecules. Here, we applied the… read more here.
Keywords: shift assay; auxin; cellular thermal; thermal shift ... See more keywords