High-Throughput Cellular Thermal Shift Assay Using Acoustic Transfer of Protein Lysates.
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DOI: 10.1021/acschembio.1c00760
Abstract: Cellular thermal shift assay (CETSA) is a valuable method to confirm target engagement within a complex cellular environment, by detecting changes in a protein's thermal stability upon ligand binding. The classical CETSA method measures changes… read more here.
Keywords: using acoustic; thermal shift; shift assay; high throughput ... See more keywords
Real-Time Cellular Thermal Shift Assay to Monitor Target Engagement
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DOI: 10.1021/acschembio.2c00334
Abstract: Determining a molecule’s mechanism of action is paramount during chemical probe development and drug discovery. The cellular thermal shift assay (CETSA) is a valuable tool to confirm target engagement in cells for a small molecule… read more here.
Keywords: cellular thermal; real time; target engagement; cetsa ... See more keywords
CETSA MS Profiling for a Comparative Assessment of FDA-Approved Antivirals Repurposed for COVID-19 Therapy Identifies TRIP13 as a Remdesivir Off-Target
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DOI: 10.1177/2472555220973597
Abstract: The reuse of preexisting small molecules for a novel emerging disease threat is a rapid measure to discover unknown applications for previously validated therapies. A pertinent and recent example where such a strategy could be… read more here.
Keywords: target; fda approved; cetsa; cetsa profiling ... See more keywords