High-Throughput Cellular Thermal Shift Assay Using Acoustic Transfer of Protein Lysates.
Sign Up to like & getrecommendations! Published in 2022 at "ACS chemical biology"
DOI: 10.1021/acschembio.1c00760
Abstract: Cellular thermal shift assay (CETSA) is a valuable method to confirm target engagement within a complex cellular environment, by detecting changes in a protein's thermal stability upon ligand binding. The classical CETSA method measures changes… read more here.
Keywords: using acoustic; thermal shift; shift assay; high throughput ... See more keywords
Real-Time Cellular Thermal Shift Assay to Monitor Target Engagement
Sign Up to like & getrecommendations! Published in 2022 at "ACS Chemical Biology"
DOI: 10.1021/acschembio.2c00334
Abstract: Determining a molecule’s mechanism of action is paramount during chemical probe development and drug discovery. The cellular thermal shift assay (CETSA) is a valuable tool to confirm target engagement in cells for a small molecule… read more here.
Keywords: cellular thermal; real time; target engagement; cetsa ... See more keywords
Abstract LB088: MS-CETSA deep functional proteomics uncovers a new induced DNA-repair programs leading to resistance to DNA-damaging drugs
Sign Up to like & getrecommendations! Published in 2025 at "Cancer Research"
DOI: 10.1158/1538-7445.am2025-lb088
Abstract: Mechanisms for resistance to cytotoxic cancer drugs are dependent on dynamic changes in the biochemistry of cellular pathways, information which is hard to obtain at the systems level. We are using a deep functional proteomics… read more here.
Keywords: cancer; damaging drugs; resistance; cetsa ... See more keywords
Abstract LB198: CETSA-driven targeted proteomics for personalized AML therapies: Investigating drug response biomarkers and resistance mechanisms
Sign Up to like & getrecommendations! Published in 2025 at "Cancer Research"
DOI: 10.1158/1538-7445.am2025-lb198
Abstract: Cancer drug resistance remains a critical challenge in cancer therapy, necessitating advanced approaches to understand and counteract this phenomenon. Acute myeloid leukemia (AML), an aggressive and heterogeneous cancer, often exhibits resistance to chemotherapy, contributing to… read more here.
Keywords: cancer; resistance mechanisms; targeted proteomics; cetsa ... See more keywords
CETSA MS Profiling for a Comparative Assessment of FDA-Approved Antivirals Repurposed for COVID-19 Therapy Identifies TRIP13 as a Remdesivir Off-Target
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DOI: 10.1177/2472555220973597
Abstract: The reuse of preexisting small molecules for a novel emerging disease threat is a rapid measure to discover unknown applications for previously validated therapies. A pertinent and recent example where such a strategy could be… read more here.
Keywords: target; fda approved; cetsa; cetsa profiling ... See more keywords
Applications of the Cellular Thermal Shift Assay to Drug Discovery in Natural Products: A Review
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DOI: 10.3390/ijms26093940
Abstract: Natural products play a crucial role in drug discovery because of their structural diversity and biological activity. However, identifying their molecular targets remains a challenge. Traditional target identification approaches such as affinity-based protein profiling and… read more here.
Keywords: natural products; cetsa; thermal shift; drug discovery ... See more keywords