Production of a high purity, C‐tagged hepatitis B surface antigen fusion protein VLP vaccine for malaria expressed in Pichia pastoris under cGMP conditions
Sign Up to like & getrecommendations! Published in 2022 at "Biotechnology and Bioengineering"
DOI: 10.1002/bit.28181
Abstract: Virus‐like particles (VLPs) induce strong humoral and cellular responses and have formed the basis of some currently licensed vaccines. Here, we present the method used for the production of R21, a VLP‐based anti‐sporozoite malaria vaccine,… read more here.
Keywords: clinical trials; cgmp; high purity; production ... See more keywords
Cyclic nucleotide selectivity of protein kinase G isozymes
Sign Up to like & getrecommendations! Published in 2020 at "Protein Science"
DOI: 10.1002/pro.4008
Abstract: The intrinsic activity of the C‐terminal catalytic (C) domain of cyclic guanosine monophosphate (cGMP)‐dependent protein kinases (PKG) is inhibited by interactions with the N‐terminal regulatory (R) domain. Selective binding of cGMP to cyclic nucleotide binding… read more here.
Keywords: cyclic nucleotide; selectivity; cnb domains; cgmp ... See more keywords
Measurement of Cyclic GMP During Plant Hypersensitive Disease Resistance Response.
Sign Up to like & getrecommendations! Published in 2018 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-7668-3_13
Abstract: Cyclic guanosine-3',5'-monophosphate (cGMP) is recognized as an important second messenger in plants, mediating intracellular signal in important physiological processes, including the hypersensitive disease resistance response induced by avirulent pathogens. In this context, the analysis of… read more here.
Keywords: resistance response; cgmp; hypersensitive disease; disease resistance ... See more keywords
A concise discussion of the regulatory role of cGMP kinase I in cardiac physiology and pathology
Sign Up to like & getrecommendations! Published in 2018 at "Basic Research in Cardiology"
DOI: 10.1007/s00395-018-0690-1
Abstract: The underlying cause of cardiac hypertrophy, fibrosis, and heart failure has been investigated in great detail using different mouse models. These studies indicated that cGMP and cGMP-dependent protein kinase type I (cGKI) may ameliorate these… read more here.
Keywords: discussion regulatory; kinase; concise discussion; pathology ... See more keywords
Ethylene promotes pollen tube growth by affecting actin filament organization via the cGMP-dependent pathway in Arabidopsis thaliana
Sign Up to like & getrecommendations! Published in 2017 at "Protoplasma"
DOI: 10.1007/s00709-017-1158-0
Abstract: Ethylene and cGMP are key regulators of plant developmental processes. In this study, we demonstrate that ethylene or cGMP promote pollen tube growth in a dose-dependent manner. The etr1–1 mutant was found to be insensitive… read more here.
Keywords: pollen tube; ethylene; cgmp; tube growth ... See more keywords
Guanosine, a guanine-based nucleoside relaxed isolated corpus cavernosum from mice through cGMP accumulation
Sign Up to like & getrecommendations! Published in 2020 at "Purinergic Signalling"
DOI: 10.1007/s11302-020-09702-5
Abstract: In corpus cavernosum (CC), guanosine triphosphate (GTP) is converted into cyclic guanosine monophosphate (cGMP) to induce erection. The action of cGMP is terminated by phosphodiesterases and efflux transporters, which pump cGMP out of the cell.… read more here.
Keywords: guanosine; corpus cavernosum; guanine based; cgmp ... See more keywords
Mg2+ Enhances the Formation of 2′,3′-cGMP, an Intermediate of RNA Cleavage by Binase
Sign Up to like & getrecommendations! Published in 2017 at "BioNanoScience"
DOI: 10.1007/s12668-016-0302-1
Abstract: Binase, the ribonuclease secreted by Bacillus pumilus, is an endonuclease that cleaves the phosphodiester bond between the 3′-guanyl residue and 5′-OH residue of an adjacent nucleotide, with the formation of a corresponding intermediate, 2′,3′-cGMP on… read more here.
Keywords: cgmp; enhances formation; binase; mg2 enhances ... See more keywords
Inhibition of iNOS alleviates cognitive deficits and depression in diabetic mice through downregulating the NO/sGC/cGMP/PKG signal pathway
Sign Up to like & getrecommendations! Published in 2017 at "Behavioural Brain Research"
DOI: 10.1016/j.bbr.2016.12.046
Abstract: Abstract Diabetes mellitus often results in a number of complications involving impaired brain function, including cognitive deficits and depression. However, the potential mechanisms for diabetes‐related cognitive deficits and depression are not fully understood. Neurons in… read more here.
Keywords: depression; cognitive deficits; deficits depression; cgmp ... See more keywords
Both nitric oxide and nitrite prevent homocysteine-induced endoplasmic reticulum stress and subsequent apoptosis via cGMP-dependent pathway in neuronal cells.
Sign Up to like & getrecommendations! Published in 2017 at "Biochemical and biophysical research communications"
DOI: 10.1016/j.bbrc.2017.09.054
Abstract: Growing evidence indicates that endoplasmic reticulum (ER) stress and/or ER stress-mediated apoptosis may play a role in the pathogenesis of neurodegenerative diseases such as Alzheimer's disease. The present study investigated the effects of non-cytotoxic concentrations… read more here.
Keywords: endoplasmic reticulum; apoptosis; reticulum stress; stress ... See more keywords
Cyclic guanosine monophosphate modulates accumulation of phosphodiesterase 5 inhibitors in human platelets
Sign Up to like & getrecommendations! Published in 2017 at "Biochemical Pharmacology"
DOI: 10.1016/j.bcp.2017.08.026
Abstract: ABSTRACT Sildenafil and tadalafil are widely‐used phosphodiesterase 5 (PDE5) inhibitors for which no clear dose‐response relationship could be established. Using isolated and/or recombinant PDE5, it has been demonstrated that cGMP can increase the affinity of… read more here.
Keywords: guanosine monophosphate; sildenafil tadalafil; tadalafil; pde5 ... See more keywords
Light-Activated Cyclic Nucleotide Phosphodiesterases
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DOI: 10.1016/j.bpj.2016.11.950
Abstract: The second messengers 3′,5′-cyclic adenosine monophosphate (cAMP) and cyclic 3′,5′-cyclic guanosine monophosphate (cGMP) mediate various biological responses like apoptosis, smooth muscle relaxation and vision. Cyclic-nucleotide-dependent processes have been analyzed with spatiotemporal resolution through optogenetics. In… read more here.
Keywords: cyclic nucleotide; light activated; phosphodiesterase; activated cyclic ... See more keywords