Gamma cyclodextrin glycosyltransferase from evansella caseinilytica: production, characterization and product specificity
Sign Up to like & getrecommendations! Published in 2021 at "3 Biotech"
DOI: 10.1007/s13205-021-03077-z
Abstract: Alkalohalophilic Evansellacaseinilytica produced an extracellular cyclodextrin glycosyltransferase (CGTase) with cyclization activity of 43.5 ± 4.4 U/L in M1 medium containing 1% starch and 6% NaCl in nutrient broth at 37 ºC, pH 9.0, after 48 h. This… read more here.
Keywords: starch; cyclodextrin glycosyltransferase; cgtase; caseinilytica ... See more keywords
Different strategies for cyclodextrin production: Ultrafiltration systems, CGTase immobilization and use of a complexing agent.
Sign Up to like & getrecommendations! Published in 2018 at "Carbohydrate polymers"
DOI: 10.1016/j.carbpol.2018.03.035
Abstract: The study comparatively evaluated diverse strategic models of cyclodextrin (CD) production by the CGTase of Bacillus firmus strain 37: continuous production and repetitive batches in ultrafiltration systems; immobilization of CGTase on curdlan and vegetable sponge… read more here.
Keywords: production; immobilization; ultrafiltration systems; cgtase ... See more keywords
High-level secretion and characterization of cyclodextrin glycosyltransferase in recombinant Komagataella phaffii.
Sign Up to like & getrecommendations! Published in 2017 at "Journal of biotechnology"
DOI: 10.1016/j.jbiotec.2017.07.031
Abstract: Cyclodextrin glycosyltransferase (CGTase) catalyzes the conversion of starch into cyclodextrin (CD), which is widely applied in food, pharmaceutical, cosmetic, and agricultural industries. For efficient production of CD, high yield of CGTase with good characteristics is… read more here.
Keywords: cyclodextrin; komagataella phaffii; cyclodextrin glycosyltransferase; cgtase ... See more keywords
Efficient secretory expression of Bacillus stearothermophilus α/β-cyclodextrin glycosyltransferase in Bacillus subtilis.
Sign Up to like & getrecommendations! Published in 2021 at "Journal of biotechnology"
DOI: 10.1016/j.jbiotec.2021.03.011
Abstract: Bacillus stearothermophilus α/β-cyclodextrin glycosyltransferase (α/β-CGTase) is an excellent transglycosylase with broad potential for food application, but its expression level is low in Bacillus subtilis. In this study, the optimal signal peptide for α/β-CGTase expression was… read more here.
Keywords: cyclodextrin glycosyltransferase; cgtase; stearothermophilus cyclodextrin; expression ... See more keywords
Medium optimization for submerged fermentative production of β‐cyclodextrin glucosyltransferase by isolated novel alkalihalophilic Bacillus sp. NCIM 5799 using statistical approach
Sign Up to like & getrecommendations! Published in 2022 at "Letters in Applied Microbiology"
DOI: 10.1111/lam.13746
Abstract: β‐cyclodextrin glucosyltransferase (β‐CGTase) is an essential enzyme to catalyse the biotransformation of starch into β‐cyclodextrins (β‐CD). β‐CD has widespread applications in the biomedical, pharmaceutical and food industries. The present study focused on β‐CGTase production using… read more here.
Keywords: cyclodextrin glucosyltransferase; ncim 5799; bacillus ncim; production ... See more keywords
Cyclodextrin enhanced the soluble expression of Bacillus clarkii γ-CGTase in Escherichia coli
Sign Up to like & getrecommendations! Published in 2018 at "BMC Biotechnology"
DOI: 10.1186/s12896-018-0480-8
Abstract: BackgroundCyclodextrin glycosyltransferases (CGTases) catalyze the synthesis of cyclodextrins, which are circular α-(1,4)-linked glucans used in many applications in the industries related to food, pharmaceuticals, cosmetics, chemicals, and agriculture, among others. Economic use of these CGTases,… read more here.
Keywords: production; escherichia coli; cyclodextrin; cgtase ... See more keywords
A two-stage temperature control strategy enhances extracellular secretion of recombinant α-cyclodextrin glucosyltransferase in Escherichia coli
Sign Up to like & getrecommendations! Published in 2017 at "AMB Express"
DOI: 10.1186/s13568-017-0465-3
Abstract: The effects of temperature on extracellular secretion of the α-cyclodextrin glucosyltransferase (α-CGTase) from Paenibacillus macerans JFB05-01 by Escherichia coli were investigated. When protein expression was induced at constant temperature, the greatest amount of extracellular recombinant… read more here.
Keywords: temperature; cyclodextrin glucosyltransferase; extracellular secretion; recombinant ... See more keywords
Effect of cyclodextrin glucosyltransferase extracted from Bacillus xiaoxiensis on wheat dough and bread properties
Sign Up to like & getrecommendations! Published in 2022 at "Frontiers in Nutrition"
DOI: 10.3389/fnut.2022.1026678
Abstract: In this study, the cyclodextrin glucosyltransferase (CGTase) was extracted from Bacillus xiaoxiensis. CGTase had negative effects on dough viscoelastic properties and gluten strength but had positive effects on bread baking qualities and anti-staling properties. Adding… read more here.
Keywords: cyclodextrin glucosyltransferase; extracted bacillus; cgtase; bread ... See more keywords
Rational Design of Cyclodextrin Glycosyltransferase with Improved Hesperidin Glycosylation Activity
Sign Up to like & getrecommendations! Published in 2023 at "Catalysts"
DOI: 10.3390/catal13050885
Abstract: Cyclodextrin glycosyltransferase (CGTase) can catalyze the glycosylation of hesperidin, resulting in α-glycosyl hesperidin with significantly improved water solubility. In this study, a rational design of CGTase to improve its hesperidin glycosylation activity was investigated. The… read more here.
Keywords: cyclodextrin glycosyltransferase; activity; glycosylation; y217f ... See more keywords
Efficient Bioconversion of Stevioside and Rebaudioside A to Glucosylated Steviol Glycosides Using an Alkalihalobacillus oshimesis-Derived Cyclodextrin Glucanotransferase
Sign Up to like & getrecommendations! Published in 2023 at "Molecules"
DOI: 10.3390/molecules28031245
Abstract: The enzymatic transglycosylation of steviol glycosides can improve the edulcorant quality of steviol glycosides. Cyclodextrin glucanotransferase (CGTase) is one of the most popular glucanotransferases applied in this reaction. Herein, the CGTase-producing strain Alkalihalobacillus oshimensis CGMCC… read more here.
Keywords: stevioside rebaudioside; steviol glycosides; glucosylated steviol; cgtase ... See more keywords