Efficient strategy for introducing large and multiple changes in plasmid DNA
Sign Up to like & getrecommendations! Published in 2018 at "Scientific Reports"
DOI: 10.1038/s41598-018-20169-8
Abstract: While the QuikChange site-directed mutagenesis method and its later modifications are extremely useful and simple, they suffer from several drawbacks. Here, we propose a new method, named LFEAP mutagenesis (Ligation of Fragment Ends After PCR)… read more here.
Keywords: introducing large; multiple changes; dna; large multiple ... See more keywords
Rapid adaptation for fibre degradation by changes in plasmid stoichiometry within Lactobacillus plantarum at the synthetic community level
Sign Up to like & getrecommendations! Published in 2020 at "Microbial Biotechnology"
DOI: 10.1111/1751-7915.13584
Abstract: The multi‐enzyme cellulosome complex can mediate the valorization of lignocellulosic biomass into soluble sugars that can serve in the production of biofuels and valuable products. A potent bacterial chassis for the production of active cellulosomes… read more here.
Keywords: adaptation fibre; rapid adaptation; degradation changes; fibre degradation ... See more keywords