Chaperone-assisted structure elucidation with DARPins.
Sign Up to like & getrecommendations! Published in 2020 at "Current opinion in structural biology"
DOI: 10.1016/j.sbi.2019.12.009
Abstract: Designed ankyrin repeat proteins (DARPins) are artificial binding proteins that have found many uses in therapy, diagnostics and biochemical research. They substantially extend the scope of antibody-derived binders. Their high affinity and specificity, rigidity, extended… read more here.
Keywords: structural biology; chaperone assisted; structure elucidation; assisted structure ... See more keywords
Chaperone-assisted selective autophagy targets filovirus VP40 as a client and restricts egress of virus particles.
Sign Up to like & getrecommendations! Published in 2023 at "Proceedings of the National Academy of Sciences of the United States of America"
DOI: 10.1073/pnas.2210690120
Abstract: The filovirus VP40 protein directs virion egress, which is regulated either positively or negatively by select VP40-host interactions. We demonstrate that host BAG3 and HSP70 recognize VP40 as a client and inhibit the egress of… read more here.
Keywords: chaperone assisted; assisted selective; vp40 client; filovirus vp40 ... See more keywords
Real-time single-molecule observation of chaperone-assisted protein folding
Sign Up to like & getrecommendations! Published in 2022 at "Science Advances"
DOI: 10.1101/2022.05.09.491114
Abstract: The ability of Hsp70 molecular chaperones to remodel the conformation of their clients is central to their biological function; however, questions remain regarding the precise molecular mechanisms by which Hsp70 machinery interacts with the client… read more here.
Keywords: protein folding; time; chaperone assisted; single molecule ... See more keywords