Fast and automated characterization of monoclonal antibody minor variants from cell cultures by combined Protein-A and multi-dimensional LC/MS methodologies.
Sign Up to like & getrecommendations! Published in 2020 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.0c01250
Abstract: Monitoring of post-translational modifications (PTMs) in therapeutic monoclonal antibodies (mAbs) is essential during their production in both upstream and downstream processes. However, characterization of PTMs using conventional peptide mapping procedure requires time-consuming and labor-intensive offline… read more here.
Keywords: automated characterization; multi dimensional; fast automated; cell ... See more keywords
Isolation and characterization of monoclonal antibodies against human carbonic anhydrase-IX.
Sign Up to like & getrecommendations! Published in 2021 at "mAbs"
DOI: 10.1080/19420862.2021.1999194
Abstract: The architectural complexity and heterogeneity of the tumor microenvironment (TME) remains a substantial obstacle in the successful treatment of cancer. Hypoxia, caused by insufficient oxygen supply, and acidosis, resulting from the expulsion of acidic metabolites,… read more here.
Keywords: isolation characterization; carbonic anhydrase; monoclonal antibodies; cancer ... See more keywords
Epitope characterization of a monoclonal antibody that selectively recognizes KIR2DL1 allotypes
Sign Up to like & getrecommendations! Published in 2022 at "Hla"
DOI: 10.1111/tan.14630
Abstract: Killer immunoglobulinâlike receptor (KIR) genes code for a family of inhibitory and activating receptors, finely tuning NK cell function. Numerous studies reported the relevance of KIR allelic polymorphism on KIR expression, ligand affinity, and strength… read more here.
Keywords: kir2dl1; kir2dl1 allotypes; kir; epitope characterization ... See more keywords
Characterization of Monoclonal Antibodies Recognizing Citrulline-Modified Residues
Sign Up to like & getrecommendations! Published in 2022 at "Frontiers in Immunology"
DOI: 10.3389/fimmu.2022.849779
Abstract: Background Citrullination is a post-translational protein modification linked to the occurrence and development of a variety of diseases. The detection of citrullinated proteins is predominately based on antibody detection although currently available reagents demonstrate detection… read more here.
Keywords: 22f1 30g2; detection; characterization monoclonal; citrulline ... See more keywords