Overexpression and characterization of a thermostable β-agarase producing neoagarotetraose from a marine isolate Microbulbifer sp. AG1
Sign Up to like & getrecommendations! Published in 2019 at "Acta Oceanologica Sinica"
DOI: 10.1007/s13131-019-1349-y
Abstract: An agarase gene containing 1 302 bp was cloned from Microbulbifer sp. AG1. It encoded a mature protein of 413 amino acids plus a 20-residue signal peptide. The recombinant enzyme without the signal peptide was… read more here.
Keywords: agarase; microbulbifer ag1; overexpression characterization; characterization thermostable ... See more keywords
Development and characterization of a thermostable GH11/GH10 xylan degrading chimeric enzyme.
Sign Up to like & getrecommendations! Published in 2021 at "Enzyme and microbial technology"
DOI: 10.1016/j.enzmictec.2021.109854
Abstract: Xylanases are categorized into different family groups, two of which are glycoside hydrolases 10 (GH10) and 11 (GH11) families. These well-characterized xylanases demonstrate different modes of action in hydrolysis of xylans. Imitating certain types of… read more here.
Keywords: chimeric enzyme; x11; thermostable gh11; enzyme ... See more keywords
Characterization of a thermostable Cas13 enzyme for one-pot detection of SARS-CoV-2
Sign Up to like & getrecommendations! Published in 2022 at "Proceedings of the National Academy of Sciences of the United States of America"
DOI: 10.1073/pnas.2118260119
Abstract: Significance Thermostable Cas13 orthologues will expand and unlock the power of CRISPR systems for synthetic biology and biotechnology applications. We identified and characterized a thermostable Cas13 orthologue from Thermoclostridium caenicola (TccCas13a), which exhibits robust cis… read more here.
Keywords: cas13; thermostable cas13; one pot; characterization thermostable ... See more keywords