Application of affinity capillary electrophoresis for charge heterogeneity profiling of biopharmaceuticals
Sign Up to like & getrecommendations! Published in 2019 at "Electrophoresis"
DOI: 10.1002/elps.201900233
Abstract: Charge heterogeneity profiling is important for the quality control (QC) of biopharmaceuticals. Because of the increasing complexity of these therapeutic entities [1], the development of alternative analytical techniques is needed. In this work, flow‐through partial‐filling… read more here.
Keywords: capillary electrophoresis; heterogeneity profiling; charge heterogeneity; electrophoresis ... See more keywords
Characterization of therapeutic mAb charge heterogeneity by iCIEF coupled to mass spectrometry (iCIEF–MS)
Sign Up to like & getrecommendations! Published in 2022 at "ELECTROPHORESIS"
DOI: 10.1002/elps.202200170
Abstract: Imaged capillary isoelectric focusing (iCIEF) has emerged as an important technique for therapeutic monoclonal antibody (mAb) charge heterogeneity analysis in the biopharmaceutical context, providing imaged detection and quantitation by UV without a mobilization step. Besides… read more here.
Keywords: charge; icief; charge heterogeneity; mab charge ... See more keywords
Ultrasensitive Characterization of Charge Heterogeneity of Therapeutic Monoclonal Antibodies Using Strong Cation Exchange Chromatography Coupled to Native Mass Spectrometry.
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DOI: 10.1021/acs.analchem.8b03773
Abstract: In therapeutic monoclonal antibody (mAb) development, charge heterogeneity of a mAb molecule is often associated with critical quality attributes and is therefore monitored throughout development and during QC release to ensure product and process consistency.… read more here.
Keywords: chromatography; charge heterogeneity; charge; exchange ... See more keywords