Productivity improvement and charge variant modulation for intensified cell culture processes by adding a carboxypeptidase B (CpB) treatment step
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DOI: 10.1002/bit.27723
Abstract: The goal of cell culture process intensification is to improve productivity while maintaining acceptable quality attributes. In this report, four processes, namely a conventional manufacturing Process A, and processes intensified by enriched N‐1 seed (Process… read more here.
Keywords: seed; productivity; treatment; process ... See more keywords
Dual‐detection approach for a charge variant analysis of monoclonal antibody combination products using imaged capillary isoelectric focusing
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DOI: 10.1002/elps.202200026
Abstract: The clinical benefits of treatments with a combination of two or more therapeutic monoclonal antibodies (mAbs) have emerged in recent years. Imaged capillary isoelectric focusing is a frequently used technology in the biopharmaceutical industry for… read more here.
Keywords: charge variant; variant analysis; combination; charge ... See more keywords
Investigation of anomalous charge variant profile reveals discrete pH-dependent conformations and conformation-dependent charge states within the CDR3 loop of a therapeutic mAb
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DOI: 10.1080/19420862.2020.1763138
Abstract: ABSTRACT During the development of a therapeutic monoclonal antibody (mAb-1), the charge variant profile obtained by pH-gradient cation exchange chromatography (CEX) contained two main peaks, each of which exhibited a unique intrinsic fluorescence profile and… read more here.
Keywords: cdr3 loop; charge; variant profile; charge variant ... See more keywords