New regulators of the tetracycline‐inducible gene expression system identified by chemical and genetic screens
Sign Up to like & getrecommendations! Published in 2022 at "FEBS Open Bio"
DOI: 10.1002/2211-5463.13482
Abstract: The tetracycline repressor (tetR)‐regulated system is a widely used tool to specifically control gene expression in mammalian cells. Based on this system, we generated a human osteosarcoma cell line, which allows for the inducible expression… read more here.
Keywords: system; expression; gene expression; new regulators ... See more keywords
Novel Micro-Phenotyping Approach to Chemical Genetic Screening for Increased Plant Tolerance to Abiotic Stress.
Sign Up to like & getrecommendations! Published in 2018 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-7874-8_2
Abstract: Studying the effects of small molecules on root system development in the context of a large-scale chemical genetic screen has previously been a technical challenge. The recent development of novel seedling growth devices ("Phytostrips"), used… read more here.
Keywords: root system; chemical genetic; chemical; abiotic stress ... See more keywords
Combined CRISPRi/a-Based Chemical Genetic Screens Reveal that Rigosertib Is a Microtubule-Destabilizing Agent
Sign Up to like & getrecommendations! Published in 2017 at "Molecular Cell"
DOI: 10.1016/j.molcel.2017.09.012
Abstract: Summary Chemical libraries paired with phenotypic screens can now readily identify compounds with therapeutic potential. A central limitation to exploiting these compounds, however, has been in identifying their relevant cellular targets. Here, we present a… read more here.
Keywords: based chemical; chemical genetic; combined crispri; genetic screens ... See more keywords
Improved Chemical-Genetic Fluorescent Markers for Live Cell Microscopy.
Sign Up to like & getrecommendations! Published in 2018 at "Biochemistry"
DOI: 10.1021/acs.biochem.8b00649
Abstract: Inducible chemical-genetic fluorescent markers are promising tools for live cell imaging requiring high spatiotemporal resolution and low background fluorescence. The fluorescence-activating and absorption shifting tag (FAST) was recently developed to form fluorescent molecular complexes with… read more here.
Keywords: live cell; microscopy; chemical genetic; fluorescent ... See more keywords
A Chemical-Genetic Approach to Generate Selective Covalent Inhibitors of Protein Kinases.
Sign Up to like & getrecommendations! Published in 2017 at "ACS chemical biology"
DOI: 10.1021/acschembio.6b01083
Abstract: Although a previously developed bump-hole approach has proven powerful in generating specific inhibitors for mapping functions of protein kinases, its application is limited by the intolerance of the large-to-small mutation by certain kinases and the… read more here.
Keywords: selective covalent; chemical genetic; protein kinases; covalent ... See more keywords
Functional Annotation of Chemical Libraries across Diverse Biological Processes
Sign Up to like & getrecommendations! Published in 2017 at "Nature chemical biology"
DOI: 10.1038/nchembio.2436
Abstract: Chemical-genetic approaches offer the potential for unbiased functional annotation of chemical libraries. Mutations can alter the response of cells to a compound, revealing chemical-genetic interactions that can elucidate a compound’s mode of action. We developed… read more here.
Keywords: annotation chemical; functional annotation; chemical genetic; biological processes ... See more keywords
Abstract IA20: Chemical-genetic interaction maps for precision therapies in breast and ovarian cancers
Sign Up to like & getrecommendations! Published in 2017 at "Molecular Cancer Therapeutics"
DOI: 10.1158/1538-8514.synthleth-ia20
Abstract: There is an urgent need in oncology to link molecular aberrations in tumors with therapeutics that can be administered in a personalized fashion. One approach identifies synthetic-lethal genetic interactions or emergent dependencies that cancer cells… read more here.
Keywords: interaction maps; chemical genetic; genetic interaction; cancer ... See more keywords
Abstract PR07: Identifying factors mediating response and resistance to chemotherapy through a chemical-genetic interaction map
Sign Up to like & getrecommendations! Published in 2017 at "Molecular Cancer Research"
DOI: 10.1158/1557-3125.dnarepair16-pr07
Abstract: Nearly every cancer patient, especially those with highly lethal and aggressive tumor types, is treated with cytotoxic chemotherapy. Currently, the selection of chemotherapy is based on average responses over a large number of patients. This… read more here.
Keywords: response; chemical genetic; map; genetic interaction ... See more keywords
Deep learning-driven prediction of drug mechanism of action from large-scale chemical-genetic interaction profiles
Sign Up to like & getrecommendations! Published in 2022 at "Journal of Cheminformatics"
DOI: 10.1186/s13321-022-00596-6
Abstract: Motivation Chemical–genetic interaction profiling is a genetic approach that quantifies the susceptibility of a set of mutants depleted in specific gene product(s) to a set of chemical compounds. With the recent advances in artificial intelligence,… read more here.
Keywords: chemical genetic; tuberculosis; large scale; chemical ... See more keywords
Predicting bioprocess targets of chemical compounds through integration of chemical-genetic and genetic interactions
Sign Up to like & getrecommendations! Published in 2018 at "PLoS Computational Biology"
DOI: 10.1371/journal.pcbi.1006532
Abstract: Chemical-genetic interactions–observed when the treatment of mutant cells with chemical compounds reveals unexpected phenotypes–contain rich functional information linking compounds to their cellular modes of action. To systematically identify these interactions, an array of mutants is… read more here.
Keywords: chemical compounds; genetic interaction; chemical genetic; genetic interactions ... See more keywords
Chemical genetic identification of GAK substrates reveals its role in regulating Na+/K+-ATPase
Sign Up to like & getrecommendations! Published in 2018 at "Life Science Alliance"
DOI: 10.26508/lsa.201800118
Abstract: Novel GAK phosphorylation targets are identified using chemical genetic methods. One of the substrates is the α subunit of the Na+/K+-ATPase, phosphorylation of which is necessary for its surface trafficking from endosomes. Conserved functions of… read more here.
Keywords: chemical genetic; gak; identification gak; role ... See more keywords