Rapid and sensitive serum glucose determination using chemical labeling coupled with black phosphorus-assisted laser desorption/ionization time-of-flight mass spectrometry.
Sign Up to like & getrecommendations! Published in 2018 at "Talanta"
DOI: 10.1016/j.talanta.2017.08.055
Abstract: Monitoring the concentration of blood glucose in patients is a key component of good medical diagnoses. Therefore, developing an accurate, rapid and sensitive strategy for monitoring blood glucose is of vital importance. We proposed a… read more here.
Keywords: glucose determination; serum; black phosphorus; serum glucose ... See more keywords
Posttranscriptional chemical labeling of RNA by using bioorthogonal chemistry.
Sign Up to like & getrecommendations! Published in 2017 at "Methods"
DOI: 10.1016/j.ymeth.2017.02.004
Abstract: Recent developments in RNA labeling technology have provided viable tools to analyze RNA synthesis, processing and function in cell-free and cellular environments. Notably, emerging methodologies based on posttranscriptional chemical labeling by using bioorthogonal chemistry have… read more here.
Keywords: rna; chemistry; chemical; posttranscriptional chemical ... See more keywords
4-Plex Chemical Labeling Strategy Based on Cinchona Alkaloid-derived Primary Amines for the Analysis of Chiral Carboxylic Acids by Liquid Chromatography-Mass Spectrometry.
Sign Up to like & getrecommendations! Published in 2019 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.9b02909
Abstract: Chiral carboxylic acids play important roles in energy metabolism and signal transduction in human body. These enantiomers usually possess different bioactivities and are also associated with the development of some diseases. Therefore, the simultaneous determination… read more here.
Keywords: chiral carboxylic; chemical labeling; carboxylic acids; carboxylic acid ... See more keywords
Dibenzocyclooctendiones (DBCDOs): Arginine-Selective Chemical Labeling Reagents Obtained through Benzilic Acid Rearrangement.
Sign Up to like & getrecommendations! Published in 2022 at "Organic letters"
DOI: 10.1021/acs.orglett.2c01970
Abstract: We demonstrate that dibenzocyclooctendiones (DBCDOs) are efficient chemical reagents for the site-specific labeling of arginine-containing biomolecules. Unlike the commonly used probes, DBCDOs undergo an irreversible ring-contracted rearrangement with the guanidinium group on arginine residues under… read more here.
Keywords: selective chemical; arginine selective; dbcdos arginine; dibenzocyclooctendiones dbcdos ... See more keywords
Chemical Labeling and Enrichment of Histone Glyoxal Adducts.
Sign Up to like & getrecommendations! Published in 2022 at "ACS chemical biology"
DOI: 10.1021/acschembio.1c00864
Abstract: Because of their long half-lives and highly nucleophilic tails, histones are particularly susceptible to accumulating nonenzymatic covalent modifications, such as glycation. The resulting modifications can have profound effects on cellular physiology due to the regulatory… read more here.
Keywords: histone; labeling enrichment; chemistry; enrichment histone ... See more keywords
Simple, Rapid Chemical Labeling and Screening of Antibodies with Luminescent Peptides
Sign Up to like & getrecommendations! Published in 2022 at "ACS Chemical Biology"
DOI: 10.1021/acschembio.2c00306
Abstract: Sensitive and selective detection assays are essential for the accurate measurement of analytes in both clinical and research laboratories. Immunoassays that rely on nonoverlapping antibodies directed against the same target analyte (e.g., sandwich enzyme-linked immunosorbent… read more here.
Keywords: rapid chemical; simple rapid; chemical; chemical labeling ... See more keywords
Selective purification and chemical labeling of a target protein on ruthenium photocatalyst-functionalized affinity beads.
Sign Up to like & getrecommendations! Published in 2017 at "Chemical communications"
DOI: 10.1039/c7cc01595j
Abstract: Selective purification and chemical labeling of a target protein in a protein mixture were simultaneously achieved on the surface of affinity beads functionalized with ligands, such as benzenesulfonamide and methotrexate (MTX), and a ruthenium complex… read more here.
Keywords: ruthenium; affinity beads; target protein; chemical ... See more keywords
Recognition-driven chemical labeling of endogenous proteins in multi-molecular crowding in live cells.
Sign Up to like & getrecommendations! Published in 2017 at "Chemical communications"
DOI: 10.1039/c7cc07177a
Abstract: Endogenous protein labeling is one of the most invaluable methods for studying the bona fide functions of proteins in live cells. However, multi-molecular crowding conditions, such as those that occur in live cells, hamper the… read more here.
Keywords: live cells; multi molecular; recognition; recognition driven ... See more keywords
Chemical labeling achieves 8-oxo-7,8-dihydroguanine mapping in the microRNA transcriptome.
Sign Up to like & getrecommendations! Published in 2023 at "Chemical communications"
DOI: 10.1039/d2cc06273a
Abstract: A labeling chemistry-based methodology, APSC-8-oxoGua-seq, is developed to sequence 8-oxoGua in the microRNA transcriptome. N-(3-Azidopropyl)-spermine-5-carboxamide (APSC) is designed for the selective labeling of 8-oxoGua, where its azide facilitates the conjugation of a cleavable linker via… read more here.
Keywords: oxo dihydroguanine; microrna transcriptome; chemical labeling; achieves oxo ... See more keywords
Chemical labeling and proteomics for characterization of unannotated small and alternative open reading frame-encoded polypeptides.
Sign Up to like & getrecommendations! Published in 2023 at "Biochemical Society transactions"
DOI: 10.1042/bst20221074
Abstract: Thousands of unannotated small and alternative open reading frames (smORFs and alt-ORFs, respectively) have recently been revealed in mammalian genomes. While hundreds of mammalian smORF- and alt-ORF-encoded proteins (SEPs and alt-proteins, respectively) affect cell proliferation,… read more here.
Keywords: alt proteins; unannotated small; seps alt; alternative open ... See more keywords