Transformation of Thioester-Initiated Star Polymers into Linear Arms via Native Chemical Ligation.
Sign Up to like & getrecommendations! Published in 2019 at "Macromolecular rapid communications"
DOI: 10.1002/marc.201900247
Abstract: The synthesis of a new class of Cu-mediated polymerization initiators with thioester functionality is demonstrated and their polymerization kinetics via single-electron transfer living radical polymerization is reported. From periodic sampling, it is found that thioester-… read more here.
Keywords: native chemical; linear arms; chemical ligation; via native ... See more keywords
Semi‐synthesis of murine prion protein by native chemical ligation and chemical activation for preparation of polypeptide‐α‐thioester
Sign Up to like & getrecommendations! Published in 2017 at "Journal of Peptide Science"
DOI: 10.1002/psc.3008
Abstract: Prions are suspected as pathogen of the fatal transmissible spongiform encephalopathies. Strategies to access homogenous prion protein (PrP) are required to fully comprehend the molecular mechanism of prion diseases. However, the polypeptide fragments from PrP… read more here.
Keywords: prion protein; chemical ligation; chemical; synthesis ... See more keywords
Oligodeoxynucleotides Modified with 2'-O-(Cysteinylaminobutyl)carbamoylethylribothymidine Residues for Native Chemical Ligation with Peptide at Internal Positions.
Sign Up to like & getrecommendations! Published in 2022 at "Bioconjugate chemistry"
DOI: 10.1021/acs.bioconjchem.1c00575
Abstract: We used native chemical ligation (NCL) to synthesize a 2'-O-{N-[N-(S-tert-butylthiocysteinyl)aminobutyl]carbamoylethyl} (CysBCE) ribothymidine-derived oligonucleotide to expand the variety of peptide conjugation sites, allowing the incorporation of peptides at the 2'-hydroxy group when the oligonucleotide forms a… read more here.
Keywords: chemical ligation; cysteinylaminobutyl carbamoylethylribothymidine; oligodeoxynucleotides modified; native chemical ... See more keywords
Orthogonal Covalent Entrapment of Cargo into Biodegradable Polymeric Micelles via Native Chemical Ligation.
Sign Up to like & getrecommendations! Published in 2022 at "Biomacromolecules"
DOI: 10.1021/acs.biomac.2c00865
Abstract: Polymeric micelles (PMs) are promising platforms for enhanced tissue targeting of entrapped therapeutic agents. Strategies to circumvent premature release of entrapped drugs include cross-linking of the micellar core as well as covalent attachment of the… read more here.
Keywords: chemical ligation; chemistry; entrapment; native chemical ... See more keywords
β-Turn Mimics by Chemical Ligation
Sign Up to like & getrecommendations! Published in 2020 at "Organic Letters"
DOI: 10.1021/acs.orglett.0c01427
Abstract: We report a simple reductive amination protocol to ligate two peptides, while simultaneously installing a β-turn mimic at the ligation junction. This strategy uses commercially available materials, mild chemical conditions, and a chemoselective ligation reaction… read more here.
Keywords: mimics chemical; chemical ligation; ligation; turn mimics ... See more keywords
Cosolvent Effects Question the Role of Water in Native Chemical Ligation.
Sign Up to like & getrecommendations! Published in 2025 at "Organic letters"
DOI: 10.1021/acs.orglett.5c04570
Abstract: Guanidine hydrochloride and acetonitrile strongly inhibit native chemical ligation (NCL). These effects, considered alongside the impact of the cosolvents on the water structure, suggest that water actively participates in NCL. read more here.
Keywords: native chemical; chemical ligation; water; cosolvent effects ... See more keywords
Bioorthogonal Chemical Ligation Creates Synthetic Antibodies with Improved Therapeutic Potency
Sign Up to like & getrecommendations! Published in 2023 at "ACS Central Science"
DOI: 10.1021/acscentsci.3c00240
Abstract: Antibodies, also known as immunoglobulins, are proteins secreted by immune cells to specifically bind foreign antigens, often from invading species. Immunoglobulin G (IgG), one of the major classes of antibodies, is composed of two heavy… read more here.
Keywords: chemical ligation; improved therapeutic; chemistry; antibodies improved ... See more keywords
Peptidoglycan-Directed Chemical Ligation for Selective Inhibition on Gram-Positive Bacteria
Sign Up to like & getrecommendations! Published in 2022 at "ACS Omega"
DOI: 10.1021/acsomega.2c06964
Abstract: Microbicides with distinct antibacterial mechanisms show potential to combat multi-drug resistance bacteria. We herein report peptidoglycan-directed chemical ligation (PGCL) between alkyne-bearing vancomycin and an azide-tagged cationic polymer. The former binds peptidoglycan and inhibits peptidoglycan crosslinking,… read more here.
Keywords: chemical ligation; gram; directed chemical; gram positive ... See more keywords
Thionoesters: A Native Chemical Ligation-Inspired Approach to Cysteine-Triggered H2S Donors.
Sign Up to like & getrecommendations! Published in 2018 at "Journal of the American Chemical Society"
DOI: 10.1021/jacs.8b07268
Abstract: Native chemical ligation (NCL) is a simple, widely used, and powerful synthetic tool to ligate N-terminal cysteine residues and C-terminal α-thioesters via a thermodynamically stable amide bond. Building on this well-established reactivity, as well as… read more here.
Keywords: native chemical; thionoesters native; cysteine; chemical ligation ... See more keywords
Single tube gene synthesis by phosphoramidate chemical ligation.
Sign Up to like & getrecommendations! Published in 2017 at "Chemical communications"
DOI: 10.1039/c7cc00858a
Abstract: Templated chemical ligation of 5'-amino and 3'-phosphate oligonucleotides was used to synthesise a 762 base pair gene for green fluorescent protein. The phosphoramidate linkage can be read by DNA polymerase and transcribed to make RNA.… read more here.
Keywords: tube gene; chemical ligation; ligation; single tube ... See more keywords
Poly(glutamic acid) hydrogels crosslinked: via native chemical ligation
Sign Up to like & getrecommendations! Published in 2017 at "New Journal of Chemistry"
DOI: 10.1039/c7nj00439g
Abstract: Mild crosslinking methods, which have a strong influence on biomedical hydrogels and scaffolds, have attracted wide attention in recent years. In this project, native chemical ligation (NCL) was utilized to prepare biocompatible and biodegradable hydrogels… read more here.
Keywords: native chemical; poly glutamic; glutamic acid; hydrogels crosslinked ... See more keywords