Chemical Proteomics Reveals Protein Tyrosination Extends Beyond the Alpha‐Tubulins in Human Cells
Sign Up to like & getrecommendations! Published in 2022 at "Chembiochem"
DOI: 10.1002/cbic.202200414
Abstract: Tubulin detyrosination-tyrosination cycle regulates the stability of microtubules. Thus far described on α-tubulins, the tyrosination level is maintained by a single tubulin-tyrosine ligase (TTL). However, the precise dynamics and tubulin isoforms which undergo (de)tyrosination in… read more here.
Keywords: tyrosine; tyrosination; reveals protein; chemical proteomics ... See more keywords
Chemical Proteomics of Host–Microbe Interactions
Sign Up to like & getrecommendations! Published in 2018 at "PROTEOMICS"
DOI: 10.1002/pmic.201700333
Abstract: The dynamic proteome plays numerous roles in the interactions of microbes—whether they are invading pathogens or symbiotic organisms—and their hosts. Host and microbe sense, respond, and manipulate each other's biology via a multitude of mechanisms,… read more here.
Keywords: proteomics host; microbe; chemical proteomics; microbe interactions ... See more keywords
Chemical proteomics reveals interactors of the alarmone diadenosine triphosphate in the cancer cell line H1299 †
Sign Up to like & getrecommendations! Published in 2022 at "Journal of Peptide Science"
DOI: 10.1002/psc.3458
Abstract: Intracellular dinucleoside polyphosphates (NpnNs) have been known for decades but the functional role remains enigmatic. Diadenosine triphosphate (Ap3A) is one of the most prominent examples, and its intercellular concentration was shown to increase upon cellular… read more here.
Keywords: cell; diadenosine triphosphate; cell line; chemical proteomics ... See more keywords
Chemical proteomics for subcellular proteome analysis.
Sign Up to like & getrecommendations! Published in 2019 at "Current opinion in chemical biology"
DOI: 10.1016/j.cbpa.2018.08.001
Abstract: Protein functions are tightly regulated by their subcellular localization and dynamic alteration. Chemical proteomics offers convenience and efficiency for profiling protein features in a native context. In this review, we summarize the recent progress of… read more here.
Keywords: proteomics subcellular; chemical proteomics; proteome analysis; subcellular proteome ... See more keywords
Drug "Clicking" on Cell-Penetrating Fluorescent Nanoparticles for In Cellulo Chemical Proteomics.
Sign Up to like & getrecommendations! Published in 2018 at "Bioconjugate chemistry"
DOI: 10.1021/acs.bioconjchem.8b00481
Abstract: Chemical proteomics approaches are widely used to identify molecular targets of existing or novel drugs. This manuscript describes the development of a straightforward approach to conjugate azide-labeled drugs via click chemistry to alkyne-tagged cell-penetrating fluorescent… read more here.
Keywords: fluorescent nanoparticles; penetrating fluorescent; chemistry; chemical proteomics ... See more keywords
Chemical Proteomics Reveals Off-Targets of the Anandamide Reuptake Inhibitor WOBE437
Sign Up to like & getrecommendations! Published in 2022 at "ACS Chemical Biology"
DOI: 10.1021/acschembio.2c00122
Abstract: Anandamide or N-arachidonoylethanolamine (AEA) is a signaling lipid that modulates neurotransmitter release via activation of the type 1 cannabinoid receptor (CB1R) in the brain. Termination of anandamide signaling is thought to be mediated via a… read more here.
Keywords: wobe437; reveals targets; inhibitor; neuro cells ... See more keywords
Chemical Proteomics Reveals Antibiotic Targets of Oxadiazolones in MRSA
Sign Up to like & getrecommendations! Published in 2022 at "Journal of the American Chemical Society"
DOI: 10.1021/jacs.2c10819
Abstract: Phenotypic screening is a powerful approach to identify novel antibiotics, but elucidation of the targets responsible for the antimicrobial activity is often challenging in the case of compounds with a polypharmacological mode of action. Here,… read more here.
Keywords: reveals antibiotic; antibiotic targets; chemical proteomics; chemical ... See more keywords
Mapping in vivo target interaction profiles of covalent inhibitors using chemical proteomics with label-free quantification.
Sign Up to like & getrecommendations! Published in 2018 at "Nature protocols"
DOI: 10.1038/nprot.2017.159
Abstract: Activity-based protein profiling (ABPP) has emerged as a valuable chemical proteomics method to guide the therapeutic development of covalent drugs by assessing their on-target engagement and off-target activity. We recently used ABPP to determine the… read more here.
Keywords: covalent inhibitors; target; protocol; chemical proteomics ... See more keywords
Chemical proteomics reveal CD147 as a functional target of pseudolaric acid B in human cancer cells.
Sign Up to like & getrecommendations! Published in 2017 at "Chemical communications"
DOI: 10.1039/c7cc04345g
Abstract: CD147 is a glycosylated transmembrane protein highly expressed on the surface of various tumor cells which plays vital roles in tumor invasion, progression and metastasis. We report the discovery of the natural product pseudolaric acid… read more here.
Keywords: reveal cd147; pseudolaric acid; functional target; chemical proteomics ... See more keywords
Cysteine-reactive probes and their use in chemical proteomics.
Sign Up to like & getrecommendations! Published in 2018 at "Chemical communications"
DOI: 10.1039/c8cc01485j
Abstract: Proteomic profiling using bioorthogonal chemical probes that selectively react with certain amino acids is now a widely used method in life sciences to investigate enzymatic activities, study posttranslational modifications and discover novel covalent inhibitors. Over… read more here.
Keywords: amino acids; cysteine reactive; chemical proteomics; use chemical ... See more keywords
Recent advances in quantitative and chemical proteomics for autophagy studies
Sign Up to like & getrecommendations! Published in 2017 at "Autophagy"
DOI: 10.1080/15548627.2017.1313944
Abstract: ABSTRACT Macroautophagy/autophagy is an evolutionarily well-conserved cellular degradative process with important biological functions that is closely implicated in health and disease. In recent years, quantitative mass spectrometry-based proteomics and chemical proteomics have emerged as important… read more here.
Keywords: advances quantitative; proteomics autophagy; chemical proteomics; autophagy studies ... See more keywords