A comparison of human mesenchymal stem cell osteogenesis in poly(ethylene glycol) hydrogels as a function of MMP‐sensitive crosslinker and crosslink density in chemically defined medium
Sign Up to like & getrecommendations! Published in 2019 at "Biotechnology and Bioengineering"
DOI: 10.1002/bit.26957
Abstract: This study investigated osteogenesis of human mesenchymal stem cells encapsulated in matrix‐metalloproteinase (MMP)‐sensitive poly(ethylene glycol) (PEG) hydrogels in chemically defined medium (10 ng/ml bone morphogenic factor‐2). Thiol‐norbornene photoclick hydrogels were formed with CRGDS and crosslinkers of… read more here.
Keywords: defined medium; mmp sensitive; osteogenesis; cell ... See more keywords
Reprogramming Human Fibroblasts to Induced Pluripotent Stem Cells Using the GFP-Marked Lentiviral Vectors in the Chemically Defined Medium.
Sign Up to like & getrecommendations! Published in 2021 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-1084-8_7
Abstract: Much investigation is needed to understand the underlying molecular mechanisms of iPSC reprogramming and to improve this technology. Lentivirus-mediated iPSC reprogramming remains the most effective method to study human pluripotency reprogramming. iPSC production is more… read more here.
Keywords: using gfp; defined medium; human fibroblasts; lentiviral vectors ... See more keywords
Generating Human iPSC-Derived Astrocytes with Chemically Defined Medium for In Vitro Disease Modeling.
Sign Up to like & getrecommendations! Published in 2019 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-9477-9_3
Abstract: To better understand and model neurological, in particular neurodegenerative diseases, human induced pluripotent stem cells (hiPSCs) offer a great source for generation of neural cells. We provide a protocol for the differentiation of hiPSc-derived astrocytes… read more here.
Keywords: vitro disease; derived astrocytes; generating human; human ipsc ... See more keywords
Phenotypic MicroArray Screening of Neisseria gonorrhoeae in Chemically Defined Liquid Medium.
Sign Up to like & getrecommendations! Published in 2019 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-9496-0_13
Abstract: Phenotype MicroArrays (PMs) provide a considerable benefit to the evaluation of potential vaccine/drug targets and the assessment of hypothetical protein function. Nearly 2000 conditions can be screened relatively quickly either to search for phenotypes associated… read more here.
Keywords: phenotypic microarray; liquid medium; phenotypic; neisseria gonorrhoeae ... See more keywords
High lipids accumulation in Rhodosporidium toruloides by applying single and multiple nutrients limitation in a simple chemically defined medium
Sign Up to like & getrecommendations! Published in 2017 at "Annals of Microbiology"
DOI: 10.1007/s13213-017-1282-2
Abstract: Microbial oil produced by the oleaginous yeast Rhodosporidium toruloides ATCC 204091 (formerly referred to as Rhodotorula glutinis) has a similar fatty acid composition to the vegetable oils and represents a potential alternative for biodiesel production.… read more here.
Keywords: accumulation; production; medium; lipid accumulation ... See more keywords
Development and application of aerobic, chemically defined media for Dysgonomonas.
Sign Up to like & getrecommendations! Published in 2020 at "Anaerobe"
DOI: 10.1016/j.anaerobe.2020.102302
Abstract: Members of Dysgonomonas are Gram-stain-negative, non-motile, facultatively anaerobic coccobacilli originally described in relation to their isolation from stool and wounds of human patients (CDC group DF-3). More recently, Dysgonomonas have been found to be widely… read more here.
Keywords: defined media; development application; media dysgonomonas; aerobic chemically ... See more keywords
Mechanically and chemically defined hydrogel matrices for patient-derived colorectal tumor organoid culture.
Sign Up to like & getrecommendations! Published in 2019 at "Biomaterials"
DOI: 10.1016/j.biomaterials.2019.119400
Abstract: Patient-derived tumor organoids offer potentially useful models of cancer tissue physiology. Yet, conventional organoid cultures utilize generic matrices that are difficult to tailor for various unique tumor microenvironments. Here, we employ synthetic, enzymatically crosslinked hydrogels… read more here.
Keywords: tumor; mechanically chemically; crc pdx; defined hydrogel ... See more keywords
Benchtop to bioreactor: T-cell culture and expansion in chemically defined media
Sign Up to like & getrecommendations! Published in 2020 at "Cytotherapy"
DOI: 10.1016/j.jcyt.2020.04.073
Abstract: Background & Aim Cell therapy is a blossoming field that focuses on the application of cells as therapeutic products; accordingly, the associated manufacturing process is cell culture. The difficulty of generating sufficient cell numbers with… read more here.
Keywords: expansion; cell culture; benchtop bioreactor; culture ... See more keywords
Chemically Defined Media Can Maintain Pig Pluripotency Network In Vitro
Sign Up to like & getrecommendations! Published in 2019 at "Stem Cell Reports"
DOI: 10.1016/j.stemcr.2019.05.028
Abstract: Summary Pig embryonic stem cells (pESCs) have been considered an important candidate for preclinical research on human therapies. However, the lack of understanding of pig pluripotent networks has hampered establishment of authentic pESCs. Here, we… read more here.
Keywords: stem cells; pig; stem; pig pluripotency ... See more keywords
A library of chemically defined human N-glycans synthesized from microbial oligosaccharide precursors
Sign Up to like & getrecommendations! Published in 2017 at "Scientific Reports"
DOI: 10.1038/s41598-017-15891-8
Abstract: Synthesis of homogenous glycans in quantitative yields represents a major bottleneck to the production of molecular tools for glycoscience, such as glycan microarrays, affinity resins, and reference standards. Here, we describe a combined biological/enzymatic synthesis… read more here.
Keywords: library chemically; glycans synthesized; human glycans; defined human ... See more keywords
Chemically-defined lactose-based autoinduction medium for site-specific incorporation of non-canonical amino acids into proteins
Sign Up to like & getrecommendations! Published in 2018 at "Rsc Advances"
DOI: 10.1039/c8ra04359k
Abstract: Optimized chemically-defined lactose-based autoinduction media for superior expression levels of proteins with non-canonical amino acids. read more here.
Keywords: based autoinduction; non canonical; defined lactose; lactose based ... See more keywords