Synthesis of a Stable Analog of the Phosphorylated Form of CheY: Phosphono-CheY.
Sign Up to like & getrecommendations! Published in 2018 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-7577-8_26
Abstract: CheY is a response regulator of bacterial chemotaxis that is activated by phosphorylation of a conserved aspartate residue. However, studies of CheY-phosphate have proven challenging due to rapid hydrolysis of the aspartyl-phosphate in vitro. To… read more here.
Keywords: synthesis stable; form; chey; analog ... See more keywords
Crowding by Poly(ethylene glycol) Destabilizes Chemotaxis Protein Y (CheY).
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DOI: 10.1021/acs.biochem.2c00030
Abstract: The prevailing understanding of various aspects of biochemical processes, including folding, stability, intermolecular interactions, and the binding of metals, substrates, and inhibitors, is derived from studies carried out under dilute and homogeneous conditions devoid of… read more here.
Keywords: magnesium; chey; poly ethylene; ethylene glycol ... See more keywords
Timescale separation in the coordinated switching of bacterial flagellar motors
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DOI: 10.1088/1478-3975/acd0fb
Abstract: The output of the bacterial chemotaxis signaling pathway, the level of the intracellular regulator CheY-P, modulates the rotation direction of the flagellar motor, thereby regulating bacterial run-and-tumble behavior. The multiple flagellar motors on an E.… read more here.
Keywords: chey; fluctuation chey; timescale separation; flagellar motors ... See more keywords
The Solvation of the E. coli CheY Phosphorylation Site Mapped by XFMS
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DOI: 10.3390/ijms232112771
Abstract: The Escherichia coli CheY protein belongs to a large bacterial response regulator superfamily. X-ray hydroxy radical foot-printing with mass spectroscopy (XFMS) has shown that allosteric activation of CheY by its motor target triggers a concerted… read more here.
Keywords: chey; solvation; site; coli chey ... See more keywords