Amphipol‐assisted purification method for the highly active and stable photosystem II supercomplex of Chlamydomonas reinhardtii
Sign Up to like & getrecommendations! Published in 2019 at "FEBS Letters"
DOI: 10.1002/1873-3468.13394
Abstract: Photosystem II (PSII) splits water and drives electron transfer to plastoquinone via photochemical reactions using light energy. It is surrounded by light‐harvesting complex II (LHCII) to form the PSII–LHCII supercomplex. Complete characterization of its structure… read more here.
Keywords: method; photosystem; amphipol assisted; chlamydomonas reinhardtii ... See more keywords
Two phosphoenolpyruvate carboxykinases with differing biochemical properties in Chlamydomonas reinhardtii
Sign Up to like & getrecommendations! Published in 2023 at "FEBS Letters"
DOI: 10.1002/1873-3468.14590
Abstract: Phosphoenolpyruvate carboxykinase (PEPCK) catalyses the reversible reaction of decarboxylation and phosphorylation of oxaloacetate (OAA) to generate phosphoenolpyruvate (PEP) and CO2 playing mainly a gluconeogenic role in green algae. We found two PEPCK isoforms in Chlamydomonas… read more here.
Keywords: carboxykinases differing; chlamydomonas reinhardtii; phosphoenolpyruvate; two phosphoenolpyruvate ... See more keywords
Promoting cell growth and characterizing partial symbiotic relationships in the co‐cultivation of green alga Chlamydomonas reinhardtii and Escherichia coli
Sign Up to like & getrecommendations! Published in 2022 at "Biotechnology Journal"
DOI: 10.1002/biot.202200099
Abstract: By co‐culturing selected microalgae and heterotrophic microorganisms, the growth rate of microalgae can be improved even under atmospheric conditions with a low CO2 concentration. However, the detailed mechanism of improvement of proliferative capacity by co‐culture… read more here.
Keywords: alga chlamydomonas; growth; green alga; escherichia coli ... See more keywords
Overexpression of the sulfate transporter‐encoding SULTR2 increases chromium accumulation in Chlamydomonas reinhardtii
Sign Up to like & getrecommendations! Published in 2023 at "Biotechnology and Bioengineering"
DOI: 10.1002/bit.28350
Abstract: Hexavalent chromium [Cr(Ⅵ)] is a highly toxic contaminant in aquatic systems, and microalgae represent promising bioremediators of metal‐containing wastewater. However, the metal‐binding capacity of algal cells is limited. Therefore, we improved the cellular Cr(Ⅵ) biosorption… read more here.
Keywords: transporter; sultr2; chlamydomonas reinhardtii; accumulation ... See more keywords
pH effects on the lipid and fatty acids accumulation in Chlamydomonas reinhardtii
Sign Up to like & getrecommendations! Published in 2019 at "Biotechnology Progress"
DOI: 10.1002/btpr.2891
Abstract: pH variations influence the delivery of essential nutrients and CO2 solubility, which impact algae metabolism. In this study the microalgal growth and chlorophyll, lipid, and fatty acids content; along with the expression of some genes… read more here.
Keywords: accumulation; effects lipid; fatty acids; lipid fatty ... See more keywords
Using YFP as a Reporter of Gene Expression in the Green Alga Chlamydomonas reinhardtii.
Sign Up to like & getrecommendations! Published in 2018 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-7724-6_10
Abstract: The unicellular green alga Chlamydomonas reinhardtii is a valuable experimental system in plant biology for studying metal homeostasis. Analyzing transcriptional regulation with promoter-fusion constructs in C. reinhardtii is a powerful method for connecting metal-responsive regulation… read more here.
Keywords: green alga; biology; chlamydomonas reinhardtii; alga chlamydomonas ... See more keywords
Long-term survival of Chlamydomonas reinhardtii during conditional senescence.
Sign Up to like & getrecommendations! Published in 2021 at "Archives of microbiology"
DOI: 10.1007/s00203-021-02508-y
Abstract: Chlamydomonas reinhardtii undergoes conditional senescence when grown in batch culture due to nutrient limitation. Here, we explored plastid and photo-physiological adaptations in Chlamydomonas reinhardtii during a long-term ageing experiment by methodically sampling them over 22 weeks.… read more here.
Keywords: chlamydomonas reinhardtii; conditional senescence; long term; senescence ... See more keywords
Rubisco mutants of Chlamydomonas reinhardtii display divergent photosynthetic parameters and lipid allocation
Sign Up to like & getrecommendations! Published in 2017 at "Applied Microbiology and Biotechnology"
DOI: 10.1007/s00253-017-8322-5
Abstract: Photosynthesis and lipid allocation were investigated in Rubisco small subunit mutants of the microalga Chlamydomonas reinhardtii. Comparative analyses were undertaken with cells grown photoheterotrophically under sulphur-replete or sulphur-depleted conditions. The Y67A Rubisco mutant, which has… read more here.
Keywords: lipid allocation; mutants chlamydomonas; rubisco mutants; rubisco ... See more keywords
Towards a biotechnological platform for the production of human pro-angiogenic growth factors in the green alga Chlamydomonas reinhardtii
Sign Up to like & getrecommendations! Published in 2019 at "Applied Microbiology and Biotechnology"
DOI: 10.1007/s00253-019-10267-6
Abstract: The recent use of photosynthetic organisms such as Chlamydomonas reinhardtii in biomedical applications has demonstrated their potential for the treatment of acute and chronic tissue hypoxia. Moreover, transgenic microalgae have been suggested as an alternative… read more here.
Keywords: human pro; pro angiogenic; growth factors; angiogenic growth ... See more keywords
Leaderless mRNAs are circularized in Chlamydomonas reinhardtii mitochondria
Sign Up to like & getrecommendations! Published in 2018 at "Current Genetics"
DOI: 10.1007/s00294-018-0848-2
Abstract: The mitochondrial genome of Chlamydomonas reinhardtii encodes eight protein coding genes transcribed on two polycistronic primary transcripts. The mRNAs are endonucleolytically cleaved from these transcripts directly upstream of their AUG start codons, creating leaderless mRNAs… read more here.
Keywords: chlamydomonas reinhardtii; reinhardtii mitochondria; circularized chlamydomonas; leaderless mrnas ... See more keywords
Co-cultivation of Chlamydomonas reinhardtii with Azotobacter chroococcum improved H2 production
Sign Up to like & getrecommendations! Published in 2017 at "Biotechnology Letters"
DOI: 10.1007/s10529-017-2301-x
Abstract: ObjectivesTo improve H2 production, the green algae Chlamydomonas reinhardtii cc849 was co-cultured with Azotobacter chroococcum.ResultsThe maximum H2 production of the co-culture was 350% greater than that of the pure algal cultures under optimal H2 production… read more here.
Keywords: chlamydomonas reinhardtii; production; azotobacter chroococcum; improved production ... See more keywords