Preparation of Staphylococcus aureus Genomic DNA Using a Chloroform Extraction and Ethanol Precipitation Method, Followed by Additional Cleanup and Quantification Steps.
Sign Up to like & getrecommendations! Published in 2023 at "Cold Spring Harbor protocols"
DOI: 10.1101/pdb.prot107899
Abstract: In this protocol, we describe the isolation of genomic DNA (gDNA) from Staphylococcus aureus strains using a chloroform extraction and ethanol precipitation method. This gDNA-isolation method is well-suited for downstream whole-genome sequencing applications when working… read more here.
Keywords: dna; genomic dna; using chloroform; chloroform extraction ... See more keywords
Automated phenol-chloroform extraction of high molecular weight genomic DNA for use in long-read single-molecule sequencing
Sign Up to like & getrecommendations! Published in 2022 at "F1000Research"
DOI: 10.12688/f1000research.109251.1
Abstract: In order to automate the genome sequencing pipeline in our laboratory, we programmed a dual-arm anthropomorphic robot, the Robotic Biology Institute’s Maholo LabDroid, to perform organic solvent-based genomic DNA extraction from cell lysates. To the… read more here.
Keywords: dna; genomic dna; extraction high; chloroform extraction ... See more keywords