Transcriptome‐Based Identification of the Optimal Reference CHO Genes for Normalisation of qPCR Data
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DOI: 10.1002/biot.201700259
Abstract: Real‐time quantitative PCR (qPCR) is the standard method for determination of relative changes in mRNA transcript abundance. Analytical accuracy, precision and reliability are critically dependent on the selection of internal control reference genes. In this… read more here.
Keywords: normalisation qpcr; reference; cho; reference genes ... See more keywords
Application of microRNA Targeted 3'UTRs to Repress DHFR Selection Marker Expression for Development of Recombinant Antibody Expressing CHO Cell Pools.
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DOI: 10.1002/biot.201800129
Abstract: The dihydrofolate reductase (DHFR) system is used for the selection of recombinant Chinese hamster ovary (CHO) cell lines using the inhibitor methotrexate (MTX). During clonal selection, endogenous DHFR expression, and resistance to MTX allows the… read more here.
Keywords: dhfr; antibody; selection; expression ... See more keywords
CRISPR/Cas12a‐mediated CHO genome engineering can be effectively integrated at multiple stages of the cell line generation process for bioproduction
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DOI: 10.1002/biot.202000308
Abstract: Most biopharmaceuticals produced today are generated using Chinese hamster ovary (CHO) cells, therefore significant attention is focused on methods to improve CHO cell productivity and product quality. The discovery of gene‐editing tools, such as CRISPR/Cas9,… read more here.
Keywords: crispr cas12a; cho; cell; cell line ... See more keywords
Coupling CRISPR interference with FACS enrichment: New approach in glycoengineering of CHO cell lines for therapeutic glycoprotein production
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DOI: 10.1002/biot.202100499
Abstract: Difficulties in obtaining and maintaining the desired level of the critical quality attributes (CQAs) of therapeutic proteins as well as the pace of the development are major challenges of current biopharmaceutical development. Therapeutic proteins, both… read more here.
Keywords: cell; facs; development; approach ... See more keywords
Cell‐controlled hybrid perfusion fed‐batch CHO cell process provides significant productivity improvement over conventional fed‐batch cultures
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DOI: 10.1002/bit.26259
Abstract: A simple method originally designed to control lactate accumulation in fed‐batch cultures of Chinese Hamster Ovary (CHO) cells has been modified and extended to allow cells in culture to control their own rate of perfusion… read more here.
Keywords: perfusion; batch cultures; fed batch; batch ... See more keywords
Karyotype variation of CHO host cell lines over time in culture characterized by chromosome counting and chromosome painting
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DOI: 10.1002/bit.26453
Abstract: Genomic rearrangements are a common phenomenon in rapidly growing cell lines such as Chinese hamster ovary (CHO) cells, a feature that in the context of production of biologics may lead to cell line and product… read more here.
Keywords: cell lines; time; cho; cell ... See more keywords
Metabolic phenotyping of CHO cells varying in cellular biomass accumulation and maintenance during fed‐batch culture
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DOI: 10.1002/bit.26485
Abstract: CHO cell lines capable of high‐level recombinant protein product biosynthesis during fed‐batch culture are still generally obtained by intensive empirical screening of transfected cells rather than knowledge‐guided cellular engineering. In this study, we investigate how… read more here.
Keywords: batch culture; fed batch; cho; cho cell ... See more keywords
Butyrated ManNAc analog improves protein expression in Chinese hamster ovary cells
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DOI: 10.1002/bit.26560
Abstract: The chemical additive sodium butyrate (NaBu) has been applied in cell culture media as a direct and convenient method to increase the protein expression in Chinese hamster ovary (CHO) and other mammalian cells. In this… read more here.
Keywords: protein expression; cell lines; treatment; expression ... See more keywords
Identification and CRISPR/Cas9 Knockout of the Endogenous C1s Protease in CHO Cells Eliminates Aberrant Proteolysis of Recombinantly Expressed Proteins.
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DOI: 10.1002/bit.27016
Abstract: Proteolysis associated with recombinant protein expression in Chinese Hamster Ovary (CHO) cells has hindered the development of biologics including HIV vaccines. When expressed in CHO cells, the recombinant HIV envelope protein, gp120, undergoes proteolytic clipping… read more here.
Keywords: proteolysis; protease; cho cells; c1s protease ... See more keywords
De novo assembly and annotation of the CHOZN® GS−/− genome supports high‐throughput genome‐scale screening
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DOI: 10.1002/bit.28226
Abstract: Chinese hamster ovary (CHO) cells have been used as the industry standard for the production of therapeutic monoclonal antibodies for several decades. Despite significant improvements in commercial‐scale production processes and media, the CHO cell has… read more here.
Keywords: novo assembly; scale; assembly annotation; cho ... See more keywords
Generation of reference cell lines, media, and a process platform for CHO cell biomanufacturing
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DOI: 10.1002/bit.28290
Abstract: Due to the favorable attributes of Chinese hamster ovary (CHO) cells for therapeutic proteins and antibodies biomanufacturing, companies generate proprietary cells with desirable phenotypes. One key attribute is the ability to stably express multi‐gram per… read more here.
Keywords: cell; platform; reference cell; cell lines ... See more keywords