Metabolic phenotyping of CHO cells varying in cellular biomass accumulation and maintenance during fed‐batch culture
Sign Up to like & getrecommendations! Published in 2018 at "Biotechnology and Bioengineering"
DOI: 10.1002/bit.26485
Abstract: CHO cell lines capable of high‐level recombinant protein product biosynthesis during fed‐batch culture are still generally obtained by intensive empirical screening of transfected cells rather than knowledge‐guided cellular engineering. In this study, we investigate how… read more here.
Keywords: batch culture; fed batch; cho; cho cell ... See more keywords
A multiauxotrophic CHO cell line for the rapid isolation of producers of diverse or high levels of recombinant proteins
Sign Up to like & getrecommendations! Published in 2022 at "Biotechnology and Bioengineering"
DOI: 10.1002/bit.28074
Abstract: Chinese Hamster Ovary (CHO) cells are widely used for the high‐level production of recombinant proteins. We created a multiauxotrophic mutant of CHO‐K1 cells, CHO8A, that is deficient in eight enzymatic steps in the purine/pyrimidine biosynthetic… read more here.
Keywords: cell; line rapid; recombinant proteins; cho cell ... See more keywords
CRISPR‐interceded CHO cell line development approaches
Sign Up to like & getrecommendations! Published in 2023 at "Biotechnology and Bioengineering"
DOI: 10.1002/bit.28329
Abstract: For industrial production of recombinant protein biopharmaceuticals, Chinese hamster ovary (CHO) cells represent the most widely adopted host cell system, owing to their capacity to produce high‐quality biologics with human‐like posttranslational modifications. As opposed to… read more here.
Keywords: line development; cell line; cell; cho cell ... See more keywords
How reliable are Chinese Hamster Ovary (CHO) cell genome-scale metabolic models?
Sign Up to like & getrecommendations! Published in 2023 at "Biotechnology and bioengineering"
DOI: 10.1002/bit.28366
Abstract: Genome-scale metabolic models (GEMs) possess the power to revolutionise bioprocess and cell line engineering workflows by their ability to predict and understand whole cell metabolism in silico. Despite this potential, it is unclear how accurately… read more here.
Keywords: scale metabolic; cell; cho cell; cho ... See more keywords
Chemical inhibitors of hexokinase-2 enzyme reduce lactate accumulation, alter glycosylation processing, and produce altered glycoforms in CHO cell cultures.
Sign Up to like & getrecommendations! Published in 2023 at "Biotechnology and bioengineering"
DOI: 10.1002/bit.28417
Abstract: Chinese hamster ovary (CHO) cells, predominant hosts for recombinant biotherapeutics production, generate lactate as a major glycolysis by-product. High lactate levels adversely impact cell growth and productivity. The goal of this study was to reduce… read more here.
Keywords: growth; cell; lactate accumulation; cho cell ... See more keywords
Probing the importance of clonality: Single cell subcloning of clonally derived CHO cell lines yields widely diverse clones differing in growth, productivity, and product quality
Sign Up to like & getrecommendations! Published in 2018 at "Biotechnology Progress"
DOI: 10.1002/btpr.2594
Abstract: In the past few decades, a large variety of therapeutic antibodies and proteins have been expressed in Chinese hamster ovary (CHO) cells. This mammalian expression system is robust, scalable, relatively inexpensive, and importantly allows for… read more here.
Keywords: single cell; cell lines; product quality; cell ... See more keywords
Fluctuations in dissolved oxygen concentration during a CHO cell culture process affects monoclonal antibody productivity and the sulfhydryl‐drug conjugation process
Sign Up to like & getrecommendations! Published in 2018 at "Biotechnology Progress"
DOI: 10.1002/btpr.2697
Abstract: During a CHO cell culture production process, important parameters are generally well controlled by a feedback mechanism (PID loop) in order to ensure consistency in both productivity and product quality. These parameters typically include pH,… read more here.
Keywords: conjugation process; process; cell culture; cho cell ... See more keywords
The enhancement of antibody concentration and achievement of high cell density CHO cell cultivation by adding nucleoside
Sign Up to like & getrecommendations! Published in 2017 at "Cytotechnology"
DOI: 10.1007/s10616-017-0066-7
Abstract: Recently, with the dramatic increase in demand for therapeutic antibodies, Chinese hamster ovary (CHO) cell culture systems have made significant progress in recombinant antibody production. Over the past two decades, recombinant antibody productivity has been… read more here.
Keywords: addition; antibody; concentration; cell ... See more keywords
Cu1+, but not Cu2+ is capable of inhibition of AQP4 permeability in an in vitro CHO cell based model
Sign Up to like & getrecommendations! Published in 2021 at "Biochemistry and Biophysics Reports"
DOI: 10.1016/j.bbrep.2021.101132
Abstract: Aquaporin 4 (AQP4) is an important water channel in the central nervous system which is implicated in several neurological disorders. Due to its significance, the identification of molecules which are able to modulate its activity… read more here.
Keywords: cu1; model; cell; cho cell ... See more keywords
Challenges to industrial mAb bioprocessing—removal of host cell proteins in CHO cell bioprocesses
Sign Up to like & getrecommendations! Published in 2018 at "Current Opinion in Chemical Engineering"
DOI: 10.1016/j.coche.2018.08.001
Abstract: Monoclonal antibodies hold a steadfast lead in the ever-expanding biologics marketplace and have revolutionized the treatment of a wide variety of illnesses. The prominence of mAbs as therapeutic agents brought with it the need for… read more here.
Keywords: host cell; mab bioprocessing; cell proteins; cell ... See more keywords
Defining lncRNAs Correlated with CHO Cell Growth and IgG Productivity by RNA-Seq
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DOI: 10.1016/j.isci.2019.100785
Abstract: Summary How the long non-coding RNA (lncRNA) genome in recombinant protein producing Chinese hamster ovary (CHO) cell lines relates to phenotype is not well described. We therefore defined the CHO cell lncRNA transcriptome from cells… read more here.
Keywords: rna seq; growth; cho cell;