An Engineered Cholesterol Oxidase Catalyses Enantioselective Oxidation of Non‐steroidal Secondary Alcohols
Sign Up to like & getrecommendations! Published in 2022 at "ChemBioChem"
DOI: 10.1002/cbic.202200075
Abstract: The enantioselective oxidation of 2° alcohols to ketones is an important reaction in synthetic chemistry, especially if it can be achieved using O2‐driven alcohol oxidases under mild reaction conditions. However to date, oxidation of secondary… read more here.
Keywords: enantioselective oxidation; oxidation; cholesterol oxidase; secondary alcohols ... See more keywords
Immobilization of Cholesterol Oxidase from Streptomyces Sp. on Magnetite Silicon Dioxide by Crosslinking Method for Cholesterol Oxidation
Sign Up to like & getrecommendations! Published in 2020 at "Applied Biochemistry and Biotechnology"
DOI: 10.1007/s12010-020-03241-w
Abstract: Enzymatic biosensor has been paid much attention to the research fields due to its advantage in medical application. As one of the application, we determined the optimum value of cholesterol oxidase against cholesterol. In this… read more here.
Keywords: cholesterol oxidase; cholesterol oxidation; cholesterol; enzyme ... See more keywords
Adsorption of cholesterol oxidase and entrapment of horseradish peroxidase in metal-organic frameworks for the colorimetric biosensing of cholesterol.
Sign Up to like & getrecommendations! Published in 2019 at "Talanta"
DOI: 10.1016/j.talanta.2019.03.060
Abstract: The co-immobilization of two enzymes onto single support commonly exhibits low efficiency due to the competition against limited sites. Water-stable metal-organic frameworks (MOFs) [i.e., PCN-333(Al)] with a high surface area and ultra-large cavities were employed… read more here.
Keywords: organic frameworks; horseradish peroxidase; cholesterol; metal organic ... See more keywords
Computational insights for the hydride transfer and distinctive roles of key residues in cholesterol oxidase
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DOI: 10.1038/s41598-017-17503-x
Abstract: Cholesterol oxidase (ChOx), a member of the glucose-methanol-choline (GMC) family, catalyzes the oxidation of the substrate via a hydride transfer mechanism and concomitant reduction of the FAD cofactor. Unlike other GMC enzymes, the conserved His447… read more here.
Keywords: cholesterol oxidase; hydride transfer; insights hydride; transfer distinctive ... See more keywords
Response Surface Methodology-Genetic Algorithm Based Medium Optimization, Purification, and Characterization of Cholesterol Oxidase from Streptomyces rimosus
Sign Up to like & getrecommendations! Published in 2018 at "Scientific Reports"
DOI: 10.1038/s41598-018-29241-9
Abstract: The applicability of the statistical tools coupled with artificial intelligence techniques was tested to optimize the critical medium components for the production of extracellular cholesterol oxidase (COD; an enzyme of commercial interest) from Streptomyces rimosus… read more here.
Keywords: methodology; cholesterol oxidase; medium; response surface ... See more keywords
Identification of cholesterol-assimilating actinomycetes strain and application of statistical modeling approaches for improvement of cholesterol oxidase production by Streptomyces anulatus strain NEAE-94
Sign Up to like & getrecommendations! Published in 2020 at "BMC Microbiology"
DOI: 10.1186/s12866-020-01775-x
Abstract: Cholesterol oxidase biosensors have been used to determine the level of cholesterol in different serum and food samples. Due to a wide range of industrial and clinical applications of microbial cholesterol oxidase, isolation and identification… read more here.
Keywords: strain neae; cholesterol oxidase; oxidase production; cholesterol ... See more keywords
Heterologous Expression and Function of Cholesterol Oxidase: A Review.
Sign Up to like & getrecommendations! Published in 2023 at "Protein and peptide letters"
DOI: 10.2174/0929866530666230525162545
Abstract: Cholesterol was first found in gallstones as an animal sterol; hence it is called cholesterol. Cholesterol oxidase is the chief enzyme in the process of cholesterol degradation. Its role is obtained by the coenzyme FAD,… read more here.
Keywords: cholesterol; heterologous expression; cholesterol oxidase; function cholesterol ... See more keywords