Measuring Activity of Cholesterol Synthesis Enzymes Using Gas Chromatography/Mass Spectrometry.
Sign Up to like & getrecommendations! Published in 2017 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-6875-6_16
Abstract: The development of gas chromatography/mass spectrometry (GC/MS) technology has improved the ease and efficiency with which sterols in biological samples can be analyzed. Its advantages include that it needs only a small amount of sample,… read more here.
Keywords: gas chromatography; mass spectrometry; cholesterol synthesis; chromatography mass ... See more keywords
Sterol Evolution: Cholesterol Synthesis in Animals Is Less a Required Trait Than an Acquired Taste
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DOI: 10.1016/j.cub.2020.06.007
Abstract: Although cholesterol is considered a vital lipid for animals, not all animals can make cholesterol. A new study employing that celebrated elegant worm, Caenorhabditis elegans, investigates mechanisms that evolved in a sterol auxotroph to enable… read more here.
Keywords: cholesterol synthesis; cholesterol; sterol evolution; sterol ... See more keywords
LDLR inhibition promotes hepatocellular carcinoma proliferation and metastasis by elevating intracellular cholesterol synthesis through the MEK/ERK signaling pathway
Sign Up to like & getrecommendations! Published in 2021 at "Molecular Metabolism"
DOI: 10.1016/j.molmet.2021.101230
Abstract: Objective Adaptive rewiring of cancer energy metabolism has received increasing attention. By binding with LDLs, LDLRs make most of the circulating cholesterol available for cells to utilize. However, it remains unclear how LDLR works in… read more here.
Keywords: mek erk; intracellular cholesterol; cholesterol synthesis; cholesterol ... See more keywords
Acetaldehyde Dehydrogenase 2 regulates HMG-CoA reductase stability and cholesterol synthesis in the liver
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DOI: 10.1016/j.redox.2021.101919
Abstract: HMG-CoA reductase (HMGCR) is the rate-limiting enzyme in cholesterol biosynthesis and the target for cholesterol-lowering therapy. Acetaldehyde dehydrogenase 2 (ALDH2) is primarily responsible for detoxifying ethanol-derived acetaldehyde and endogenous lipid aldehydes derived from lipid peroxidation.… read more here.
Keywords: acetaldehyde dehydrogenase; hmg coa; cholesterol synthesis; cholesterol ... See more keywords
Disrupting Hepatocyte Cyp51 from Cholesterol Synthesis Leads to Progressive Liver Injury in the Developing Mouse and Decreases RORC Signalling
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DOI: 10.1038/srep40775
Abstract: Development of mice with hepatocyte knockout of lanosterol 14α-demethylase (HCyp51−/−) from cholesterol synthesis is characterized by the progressive onset of liver injury with ductular reaction and fibrosis. These changes begin during puberty and are generally… read more here.
Keywords: liver injury; cyp51; hepatocyte; cholesterol synthesis ... See more keywords
A key mammalian cholesterol synthesis enzyme, squalene monooxygenase, is allosterically stabilized by its substrate
Sign Up to like & getrecommendations! Published in 2020 at "Proceedings of the National Academy of Sciences of the United States of America"
DOI: 10.1073/pnas.1915923117
Abstract: Significance Cholesterol is an essential component of cell membranes and a precursor for steroid hormones and bile acids. Squalene monooxygenase (SM) is a rate-limiting enzyme in the cholesterol biosynthetic pathway and has been demonstrated to… read more here.
Keywords: cholesterol synthesis; squalene monooxygenase; cholesterol; squalene ... See more keywords
Pharmacological effects of Artocarpus lakoocha methanol extract on inhibition of squalene synthase and other downstream enzymes of the cholesterol synthesis pathway
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DOI: 10.1080/13880209.2022.2063346
Abstract: Abstract Context Artocarpus lakoocha Roxb. (Moraceae) is reported to possess antioxidant, anti-inflammatory, and anti-skin ageing agents. Objective This study evaluates the pharmacological effects of A. lakoocha leaves methanol extract on enzymes involved in the cholesterol… read more here.
Keywords: artocarpus lakoocha; methanol extract; cholesterol synthesis; synthesis pathway ... See more keywords
Hypoxia truncates and constitutively activates the key cholesterol synthesis enzyme squalene monooxygenase
Sign Up to like & getrecommendations! Published in 2022 at "eLife"
DOI: 10.1101/2022.08.18.504470
Abstract: Cholesterol synthesis is both energy- and oxygen-intensive, yet relatively little is known of the regulatory effects of hypoxia on pathway enzymes. We previously showed that the rate-limiting and first oxygen-requiring enzyme of the committed cholesterol… read more here.
Keywords: squalene monooxygenase; cholesterol; cholesterol synthesis;
Augmentation of 3β-hydroxysteroid-Δ24 Reductase (DHCR24) Expression Induced by Bovine Viral Diarrhea Virus Infection Facilitates Viral Replication via Promoting Cholesterol Synthesis
Sign Up to like & getrecommendations! Published in 2022 at "Journal of Virology"
DOI: 10.1128/jvi.01492-22
Abstract: Bovine viral diarrhea virus (BVDV), an important pathogen of cattle, is the causative agent of bovine viral diarrhea-mucosal disease, which causes extensive economic losses in both cow- and beef-rearing industry worldwide. The molecular interactions between… read more here.
Keywords: viral diarrhea; replication; cholesterol synthesis; cholesterol ... See more keywords
Cholesterol Synthesis Increased in the MMI-Induced Subclinical Hypothyroidism Mice Model
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DOI: 10.1155/2017/7921071
Abstract: Subclinical hypothyroidism (SCH) is defined as increased serum thyroid-stimulating hormone (TSH) concentrations and normal serum thyroid hormone (TH) levels as well as an increased serum cholesterol level, which is an important cause of secondary hypercholesterolemia… read more here.
Keywords: cholesterol synthesis; cholesterol; model; mmi ... See more keywords
Bilirubin ameliorates murine atherosclerosis through inhibiting cholesterol synthesis and reshaping the immune system
Sign Up to like & getrecommendations! Published in 2022 at "Journal of Translational Medicine"
DOI: 10.1186/s12967-021-03207-4
Abstract: Atherosclerosis is a chronic inflammatory disease caused mainly by lipid accumulation and excessive inflammatory immune response. Although the lipid-lowering and cardioprotective properties of bilirubin, as well as the negative relationship between bilirubin and atherosclerosis, were… read more here.
Keywords: atherosclerosis inhibiting; bilirubin; cholesterol; atherosclerosis ... See more keywords