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Published in 2021 at "Cartilage"
DOI: 10.1177/19476035211057242
Abstract: OBJECTIVE The classic chondrocyte isolation protocol is a 1-step enzymatic digestion protocol in which cartilage samples are digested in collagenase solution for a single, long period. However, this method usually results in incomplete cartilage dissociation…
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Keywords:
protocol;
chondrocyte isolation;
digestion;
cartilage ... See more keywords
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Published in 2022 at "Frontiers in Bioengineering and Biotechnology"
DOI: 10.3389/fbioe.2022.1046127
Abstract: The isolation of chondrocytes from human articular cartilage for single-cell RNA sequencing requires extensive and prolonged tissue digestion at 37 C. Modulations of the transcriptional activity likely take place during this period such that the…
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Keywords:
chondrocyte;
chondrocyte isolation;
human articular;
isolation ... See more keywords