Sequential Enzymatic Digestion of Different Cartilage Tissues: A Rapid and High-Efficiency Protocol for Chondrocyte Isolation, and Its Application in Cartilage Tissue Engineering.
Sign Up to like & getrecommendations! Published in 2021 at "Cartilage"
DOI: 10.1177/19476035211057242
Abstract: OBJECTIVE The classic chondrocyte isolation protocol is a 1-step enzymatic digestion protocol in which cartilage samples are digested in collagenase solution for a single, long period. However, this method usually results in incomplete cartilage dissociation… read more here.
Keywords: protocol; chondrocyte isolation; digestion; cartilage ... See more keywords
Optimization of chondrocyte isolation from human articular cartilage to preserve the chondrocyte transcriptome
Sign Up to like & getrecommendations! Published in 2022 at "Frontiers in Bioengineering and Biotechnology"
DOI: 10.3389/fbioe.2022.1046127
Abstract: The isolation of chondrocytes from human articular cartilage for single-cell RNA sequencing requires extensive and prolonged tissue digestion at 37 C. Modulations of the transcriptional activity likely take place during this period such that the… read more here.
Keywords: chondrocyte; chondrocyte isolation; human articular; isolation ... See more keywords