Nuclei Isolation Staining (NIS) Method for Imaging Chromatin‐Associated Proteins in Difficult Cell Types
Sign Up to like & getrecommendations! Published in 2019 at "Current Protocols in Cell Biology"
DOI: 10.1002/cpcb.94
Abstract: Spatial distribution of chromatin‐associated proteins provides invaluable information for understanding gene regulation. Conventional immunostaining is widely used for labeling chromatin‐associated proteins in many cell types. However, for a subset of difficult cell types, such as… read more here.
Keywords: associated proteins; cell; chromatin associated; nis method ... See more keywords
Chromatin‐associated noncoding RNAs in development and inheritance
Sign Up to like & getrecommendations! Published in 2017 at "Wiley Interdisciplinary Reviews: RNA"
DOI: 10.1002/wrna.1435
Abstract: Noncoding RNAs (ncRNAs) have emerged as crucial players in chromatin regulation. Their diversity allows them to partake in the regulation of numerous cellular processes across species. During development, long and short ncRNAs act in conjunction… read more here.
Keywords: epigenetic marks; inheritance; chromatin associated; noncoding rnas ... See more keywords
RChIP-Seq: Chromatin-Associated RNA Sequencing in Developmentally Staged Mouse Testes.
Sign Up to like & getrecommendations! Published in 2018 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-8663-7_9
Abstract: Chromatin is a dynamic macromolecular structure comprised of histones and a wealth of non-histone proteins. Recently, it has become clear that RNA is also an integral component of chromatin playing an important role in regulating… read more here.
Keywords: chromatin; rchip seq; chromatin associated; seq chromatin ... See more keywords
Chromatin-Associated Membraneless Organelles in Regulation of Cellular Differentiation
Sign Up to like & getrecommendations! Published in 2020 at "Stem Cell Reports"
DOI: 10.1016/j.stemcr.2020.10.011
Abstract: Membrane-free intracellular biocondensates are enclosures of proteins and nucleic acids that form by phase separation. Extensive ensembles of nuclear “membraneless organelles” indicate their involvement in genome regulation. Indeed, nuclear bodies have been linked to regulation… read more here.
Keywords: associated membraneless; membraneless organelles; membraneless; differentiation ... See more keywords
A chemical method for genome- and proteome-wide enrichment and O-GlcNAcylation profiling of chromatin-associated proteins.
Sign Up to like & getrecommendations! Published in 2021 at "Talanta"
DOI: 10.1016/j.talanta.2021.123167
Abstract: O-Linked β-N-acetylglucosamine (O-GlcNAc), a versatile posttranslational modification (PTM), is found on many chromatin-associated proteins (CAPs), such as transcription factors and their cofactors (TFCs). O-GlcNAc turnover influences the dynamic interactions of CAPs with chromatin and thereby… read more here.
Keywords: dna; chromatin; glcnacylation; associated proteins ... See more keywords
A new chemical approach for proximity labelling of chromatin-associated RNAs and proteins with visible light irradiation.
Sign Up to like & getrecommendations! Published in 2019 at "Chemical communications"
DOI: 10.1039/c9cc06251c
Abstract: A new nucleus-localized singlet oxygen generator was designed and synthesized. Its capability to label nucleus-localized biomolecules through spatially-restricted oxidation under visible light was validated using fluorescence confocal microscopy. Western blot and RT-qPCR were performed to… read more here.
Keywords: new chemical; visible light; chromatin associated; associated rnas ... See more keywords
Chromatin-contact atlas reveals disorder-mediated protein interactions and moonlighting chromatin-associated RBPs
Sign Up to like & getrecommendations! Published in 2021 at "Nucleic Acids Research"
DOI: 10.1093/nar/gkab1180
Abstract: Abstract RNA-binding proteins (RBPs) play diverse roles in regulating co-transcriptional RNA-processing and chromatin functions, but our knowledge of the repertoire of chromatin-associated RBPs (caRBPs) and their interactions with chromatin remains limited. Here, we developed SPACE… read more here.
Keywords: chromatin; chromatin contact; associated rbps; chromatin associated ... See more keywords
The chromatin-associated Sin3B protein is required for hematopoietic stem cell functions in mice.
Sign Up to like & getrecommendations! Published in 2017 at "Blood"
DOI: 10.1182/blood-2016-06-721746
Abstract: Hematopoietic stem cells (HSCs) reside at the top of the hematopoietic hierarchy and are the origin of all blood cells produced throughout an individual's life. The balance between HSC self-renewal and differentiation is maintained by… read more here.
Keywords: sin3b protein; chromatin associated; hematopoietic stem; associated sin3b ... See more keywords
Quantifying the phase separation property of chromatin-associated proteins under physiological conditions using an anti-1,6-hexanediol index
Sign Up to like & getrecommendations! Published in 2021 at "Genome Biology"
DOI: 10.1186/s13059-021-02456-2
Abstract: Background Liquid-liquid phase separation (LLPS) is an important organizing principle for biomolecular condensation and chromosome compartmentalization. However, while many proteins have been reported to undergo LLPS, quantitative and global analysis of chromatin LLPS property remains… read more here.
Keywords: phase separation; chromatin; associated proteins; chromatin associated ... See more keywords
Cystatin B deficiency results in sustained histone H3 tail cleavage in postnatal mouse brain mediated by increased chromatin-associated cathepsin L activity
Sign Up to like & getrecommendations! Published in 2022 at "Frontiers in Molecular Neuroscience"
DOI: 10.3389/fnmol.2022.1069122
Abstract: Cystatin B (CSTB) is a cysteine cathepsin inhibitor whose biallelic loss-of-function mutations in human result in defects in brain development and in neurodegeneration. The physiological function of CSTB is largely unknown, and the mechanisms underlying… read more here.
Keywords: cathepsin; chromatin associated; brain; postnatal mouse ... See more keywords