Chromatin Immunoprecipitation of Histone Modifications in Fission Yeast.
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DOI: 10.1007/978-1-4939-6630-1_12
Abstract: Chromatin immunoprecipitation (ChIP) is a sensitive, accurate, and reliable technique widely used to analyze protein-DNA interactions at specific binding sites in vivo. It has been a particularly powerful technique for mapping of histone modification patterns… read more here.
Keywords: fission yeast; histone modifications; histone; chromatin immunoprecipitation ... See more keywords
High-Resolution Chromatin Immunoprecipitation: ChIP-Sequencing.
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DOI: 10.1007/978-1-4939-7098-8_6
Abstract: Chromatin immunoprecipitation (ChIP) coupled with next-generation sequencing (NGS) is widely used for studying the nucleoprotein components that are involved in the various cellular processes required for shaping the bacterial nucleoid. This methodology, termed ChIP-sequencing (ChIP-seq),… read more here.
Keywords: high resolution; chip sequencing; immunoprecipitation chip; chromatin immunoprecipitation ... See more keywords
Native Chromatin Immunoprecipitation-Sequencing (ChIP-Seq) from Low Cell Numbers.
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DOI: 10.1007/978-1-4939-7380-4_14
Abstract: ChIP-seq is the current method of choice for genome-wide protein location analysis. Here, we present a native (non-cross-linked) ChIP procedure suitable for histone proteins, coupled with an efficient library preparation technique for subsequent next-generation sequencing.… read more here.
Keywords: chip seq; sequencing chip; chip; native chromatin ... See more keywords
Chromatin Immunoprecipitation (ChIP) with Erythroid Samples.
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DOI: 10.1007/978-1-4939-7428-3_13
Abstract: Chromatin immunoprecipitation (ChIP) allows determination of the locations to which a select protein is bound in chromatin. Chemical crosslinking of DNA and protein with bi-functional reagents such as formaldehyde and precipitation of the protein with… read more here.
Keywords: immunoprecipitation chip; erythroid samples; chromatin immunoprecipitation; chip erythroid ... See more keywords
Chromatin Immunoprecipitation-Polymerase Chain Reaction (ChIP-PCR) Detects Methylation, Acetylation, and Ubiquitylation in S. pombe.
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DOI: 10.1007/978-1-4939-7546-4_3
Abstract: The distribution of modified histones within the fission yeast Schizosaccharomyces pombe genome is ultimately dependent upon the transcriptional activity and in turn influences the ability of the polymerases to bind and progress through the chromatin… read more here.
Keywords: chromatin; reaction chip; immunoprecipitation polymerase; chain reaction ... See more keywords
Chromatin Immunoprecipitation in Early Mouse Embryos.
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DOI: 10.1007/978-1-4939-7714-7_14
Abstract: Epigenetic regulation is achieved at many levels by different factors such as tissue-specific transcription factors, members of the basal transcriptional apparatus, chromatin-binding proteins, and noncoding RNAs. Importantly, chromatin structure dictates the availability of a specific… read more here.
Keywords: chromatin; mouse embryos; chromatin immunoprecipitation; mouse ... See more keywords
Chromatin Immunoprecipitation Sequencing (ChIP-Seq) for Transcription Factors and Chromatin Factors in Arabidopsis thaliana Roots: From Material Collection to Data Analysis.
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DOI: 10.1007/978-1-4939-7747-5_18
Abstract: Chromatin immunoprecipitation combined with next-generation sequencing (ChIP-seq) is a powerful technique to investigate in vivo transcription factor (TF) binding to DNA, as well as chromatin marks. Here we provide a detailed protocol for all the… read more here.
Keywords: chip seq; chromatin; sequencing chip; chromatin immunoprecipitation ... See more keywords
Chromatin Immunoprecipitation and High-Throughput Sequencing (ChIP-Seq): Tips and Tricks Regarding the Laboratory Protocol and Initial Downstream Data Analysis.
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DOI: 10.1007/978-1-4939-7774-1_15
Abstract: Chromatin immunoprecipitation coupled with high-throughput sequencing (ChIP-seq) has become an essential tool for epigenetic scientists. ChIP-seq is used to map protein-DNA interactions and epigenetic marks such as histone modifications at the genome-wide level. Here we… read more here.
Keywords: chip seq; chip; sequencing chip; throughput sequencing ... See more keywords
The Use of the Chromatin Immunoprecipitation Technique for In Vivo Identification of Plant Protein-DNA Interactions.
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DOI: 10.1007/978-1-4939-7871-7_23
Abstract: Two-hybrid systems allow for the identification of proteins that physically interact in the context of biological processes. In the cases where these proteins interact with DNA it is essential to define their binding properties with… read more here.
Keywords: identification; protein dna; chromatin immunoprecipitation; dna ... See more keywords
Chromatin Immunoprecipitation for Identification of Protein-DNA Interactions in Human Cells.
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DOI: 10.1007/978-1-4939-7871-7_24
Abstract: Chromatin immunoprecipitation (ChIP) is a powerful technique allowing for investigation of protein-DNA interactions in living cells. Here, we provide a detailed step-by-step protocol for ChIP and highlight important considerations, challenges and pitfalls often encountered in… read more here.
Keywords: protein dna; dna interactions; chromatin immunoprecipitation; chip ... See more keywords
Chromatin Immunoprecipitation (ChIP) Protocols for the Cancer and Developmental Biology Laboratory.
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DOI: 10.1007/978-1-4939-8805-1_13
Abstract: Chromatin immunoprecipitation assays permit the isolation and subsequent identification of genomic DNA (gDNA) fragments bound directly or indirectly to proteins of interest, including transcription factors, co-factors, or chromatin remodeling proteins. These isolated DNA fragments may… read more here.
Keywords: cancer; biology; chromatin immunoprecipitation; chip ... See more keywords