Purification of cell-derived japanese encephalitis virus by dual-mode chromatography.
Sign Up to like & getrecommendations! Published in 2020 at "Biotechnology and applied biochemistry"
DOI: 10.1002/bab.1960
Abstract: Purification of the enveloped virus poses a challenge as one must retain viral infectivity to preserve immunogenicity. The traditional process of virus purification is time-consuming, laborious and hard to scaleup. Here, a rapid, simple and… read more here.
Keywords: chromatography; japanese encephalitis; purification; encephalitis virus ... See more keywords
Optimized Continuous Multicolumn Chromatography Enables Increased Productivities and Cost Savings by Employing More Columns
Sign Up to like & getrecommendations! Published in 2019 at "Biotechnology Journal"
DOI: 10.1002/biot.201800179
Abstract: The advantages of continuous chromatography with respect to increased capacity are well established. However, the impact of different loading scenarios and total number of columns on the process economics has not been addressed. Here four… read more here.
Keywords: chromatography; multicolumn chromatography; process; continuous multicolumn ... See more keywords
Mimicking continuous capture chromatography for virus clearance using a single chromatography column model
Sign Up to like & getrecommendations! Published in 2022 at "Biotechnology Journal"
DOI: 10.1002/biot.202100433
Abstract: Continuous chromatography is increasingly being used across the biotechnology industry due to its economic advantages. For adoption in commercial manufacturing, also models for virus clearance studies must be available. It is demonstrated how for a… read more here.
Keywords: virus; capture chromatography; model; virus clearance ... See more keywords
Impact of micro and macroporous TFF membranes on product sieving and chromatography loading for perfusion cell culture
Sign Up to like & getrecommendations! Published in 2019 at "Biotechnology and Bioengineering"
DOI: 10.1002/bit.27192
Abstract: Bioprocess intensification can be achieved through high cell density perfusion cell culture with continuous protein capture integration. Protein passage and cell retention are commonly accomplished using tangential flow filtration systems consisting of microporous membranes. Significant… read more here.
Keywords: product sieving; chromatography; cell culture; perfusion cell ... See more keywords
Purification processes of live virus vaccine candidates expressed in adherent Vero cell lines via multimodal chromatography in flowthrough mode.
Sign Up to like & getrecommendations! Published in 2023 at "Biotechnology and bioengineering"
DOI: 10.1002/bit.28430
Abstract: Live virus vaccine (LVV) purification, employing chromatography, can be challenged by low binding capacities and elution yields. Alternatively, processes relying solely on enzymatic digestion steps and size-based membrane separations can be limited by suboptimal reduction… read more here.
Keywords: purification; cell; live virus; chromatography ... See more keywords
Predictive scaling of fiber-based protein A capture chromatography using mechanistic modeling.
Sign Up to like & getrecommendations! Published in 2023 at "Biotechnology and bioengineering"
DOI: 10.1002/bit.28434
Abstract: Protein A affinity chromatography is an important step in the purification of monoclonal antibodies (mAbs) and mAb-derived biotherapeutics. While the biopharma industry has extensive expertise in the operation of protein A chromatography, the mechanistic understanding… read more here.
Keywords: adsorption; fiber based; based protein; chromatography ... See more keywords
Effect of guanidine and arginine on protein–ligand interactions in multimodal cation‐exchange chromatography
Sign Up to like & getrecommendations! Published in 2017 at "Biotechnology Progress"
DOI: 10.1002/btpr.2419
Abstract: The addition of fluid phase modifiers provides significant opportunities for increasing the selectivity of multimodal chromatography. In order to optimize this selectivity, it is important to understand the fundamental interactions between proteins and these modifiers.… read more here.
Keywords: guanidine; guanidine arginine; effect guanidine; chromatography ... See more keywords
Mechanistic modeling based process analytical technology implementation for pooling in hydrophobic interaction chromatography
Sign Up to like & getrecommendations! Published in 2019 at "Biotechnology Progress"
DOI: 10.1002/btpr.2758
Abstract: A major challenge in chromatography purification of therapeutic proteins is batch‐to‐batch variability with respect to impurity levels and product concentration in the feed. Mechanistic model can enable process analytical technology (PAT) implementation by predicting impact… read more here.
Keywords: chromatography; hydrophobic interaction; analytical technology; model ... See more keywords
FcγRIIIA affinity chromatography complements conventional functional characterization of rituximab
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DOI: 10.1002/btpr.3304
Abstract: Analytical and functional characterization of batches of biologics/biosimilar products are imperative towards qualifying them for pre‐clinical and clinical investigations. Several orthogonal strategies are employed to characterize the functional attributes of these drugs. However, the use… read more here.
Keywords: affinity chromatography; chromatography; riiia affinity; functional characterization ... See more keywords
Mechanistic modeling, simulation, and optimization of mixed‐mode chromatography for an antibody polishing step
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DOI: 10.1002/btpr.3316
Abstract: Mixed‐mode chromatography combines features of ion‐exchange chromatography and hydrophobic interaction chromatography and is increasingly used in antibody purification. As a replacement for flow‐through operations on traditional unmixed resins or as a pH‐controlled bind‐and‐elute step, the… read more here.
Keywords: step; mode chromatography; process; mixed mode ... See more keywords
Evaluating Multi-Product Chromatography Protein A Reuse for Monoclonal Antibodies In Biopharmaceutical Manufacturing.
Sign Up to like & getrecommendations! Published in 2023 at "Biotechnology progress"
DOI: 10.1002/btpr.3333
Abstract: In good manufacturing practice (GMP) facilities in the biopharmaceutical industry, chromatography resins are largely underutilized during purification of single drug products during clinical production. Chromatography resins are dedicated to a specific product and disposed of,… read more here.
Keywords: product; carryover; chromatography; monoclonal antibodies ... See more keywords