Using Pulsed-Field Gel Electrophoresis to Analyze Schizosaccharomyces pombe Chromosomes and Chromosomal Elements.
Sign Up to like & getrecommendations! Published in 2018 at "Cold Spring Harbor protocols"
DOI: 10.1101/pdb.prot092023
Abstract: Pulsed field gel electrophoresis (PFGE) uses alternatively oriented pulsed electrical fields to separate large DNA molecules. Here, we describe PFGE protocols and conditions for separating and visualizing chromosomes between 0.5 and 6 Mb (optimal for… read more here.
Keywords: chromosomal elements; pulsed field; field gel; gel electrophoresis ... See more keywords