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Published in 2020 at "Cold Spring Harbor protocols"
DOI: 10.1101/pdb.prot098095
Abstract: In the one-step approach to bacterial artificial chromosome (BAC) modification, two plasmids are introduced into the BAC host cells. The shuttle pLD53.SC2, carrying the EFGP reporter sequence and requiring the π protein to replicate, must…
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Keywords:
bac;
bac modification;
one step;
chromosome bac ... See more keywords