A microfluidic device for isolating intact chromosomes from single mammalian cells and probing their folding stability by controlling solution conditions
Sign Up to like & getrecommendations! Published in 2018 at "Scientific Reports"
DOI: 10.1038/s41598-018-31975-5
Abstract: Chromatin folding shows spatio-temporal fluctuations in living undifferentiated cells, but fixed spatial heterogeneity in differentiated cells. However, little is known about variation in folding stability along the chromatin fibres during differentiation. In addition, effective methods… read more here.
Keywords: folding stability; single mammalian; microfluidic device; chromosomes single ... See more keywords
Capture of complete ciliate chromosomes in single sequencing reads reveals widespread chromosome isoforms
Sign Up to like & getrecommendations! Published in 2019 at "BMC Genomics"
DOI: 10.1186/s12864-019-6189-9
Abstract: BackgroundWhole-genome shotgun sequencing, which stitches together millions of short sequencing reads into a single genome, ushered in the era of modern genomics and led to a rapid expansion of the number of genome sequences available.… read more here.
Keywords: chromosome isoforms; single sequencing; sequencing reads; chromosomes single ... See more keywords