Germ cell regeneration-mediated, enhanced mutagenesis in the ascidian Ciona intestinalis reveals flexible germ cell formation from different somatic cells.
Sign Up to like & getrecommendations! Published in 2017 at "Developmental biology"
DOI: 10.1016/j.ydbio.2017.01.022
Abstract: The ascidian Ciona intestinalis has a high regeneration capacity that enables the regeneration of artificially removed primordial germ cells (PGCs) from somatic cells. We utilized PGC regeneration to establish efficient methods of germ line mutagenesis… read more here.
Keywords: ciona; mutagenesis; regeneration; germ cell ... See more keywords
The Ciona myogenic regulatory factor functions as a typical MRF but possesses a novel N-terminus that is essential for activity.
Sign Up to like & getrecommendations! Published in 2019 at "Developmental biology"
DOI: 10.1016/j.ydbio.2018.10.010
Abstract: Electroporation-based assays were used to test whether the myogenic regulatory factor (MRF) of Ciona intestinalis (CiMRF) interferes with endogenous developmental programs, and to evaluate the importance of its unusual N-terminus for muscle development. We found… read more here.
Keywords: ciona; regulatory factor; mrf; activity ... See more keywords
The nervous system of the adult ascidian Ciona intestinalis Type A (Ciona robusta): Insights from transgenic animal models
Sign Up to like & getrecommendations! Published in 2017 at "PLoS ONE"
DOI: 10.1371/journal.pone.0180227
Abstract: The nervous system of ascidians is an excellent model system to provide insights into the evolutionary process of the chordate nervous system due to their phylogenetic positions as the sister group of vertebrates. However, the… read more here.
Keywords: ciona; system; nervous system; innervation ... See more keywords
A Manually Curated Gene Model Set for an Ascidian, Ciona robusta (Ciona intestinalis Type A)
Sign Up to like & getrecommendations! Published in 2022 at "Zoological Science"
DOI: 10.2108/zs210102
Abstract: Gene/transcript model sets predicted from decoded genome sequences are an important resource for a wide range of biological studies. Accuracy of gene models is therefore critical for deducing accurate conclusions. Computationally predicted models are sometimes… read more here.
Keywords: model set; gene transcript; ciona; transcript model ... See more keywords
Conserved Amino Acids Residing Outside the Voltage Field Can Shift the Voltage Sensitivity and Increase the Signal Speed and Size of Ciona Based GEVIs
Sign Up to like & getrecommendations! Published in 2022 at "Frontiers in Cell and Developmental Biology"
DOI: 10.3389/fcell.2022.868143
Abstract: To identify potential regions of the voltage-sensing domain that could shift the voltage sensitivity of Ciona intestinalis based Genetically Encoded Voltage Indicators (GEVIs), we aligned the amino acid sequences of voltage-gated sodium channels from different… read more here.
Keywords: amino acids; ciona; voltage sensing; voltage ... See more keywords
Matrix metalloproteinase Nas15 regulates the lumen formation and expansion in Ciona notochord
Sign Up to like & getrecommendations! Published in 2024 at "Frontiers in Ecology and Evolution"
DOI: 10.3389/fevo.2024.1385516
Abstract: Lumen formation, as a key process of biological tube construction, is essential in various physiological processes such as nutrient and waste transporting, gas exchanging, and structural supporting. However, the mechanisms underlying tubular lumen development are… read more here.
Keywords: metalloproteinase; notochord; nas15; ciona ... See more keywords
Ebf Activates Expression of a Cholinergic Locus in a Multipolar Motor Ganglion Interneuron Subtype in Ciona
Sign Up to like & getrecommendations! Published in 2021 at "Frontiers in Neuroscience"
DOI: 10.3389/fnins.2021.784649
Abstract: Conserved transcription factors termed “terminal selectors” regulate neuronal sub-type specification and differentiation through combinatorial transcriptional regulation of terminal differentiation genes. The unique combinations of terminal differentiation gene products in turn contribute to the functional identities… read more here.
Keywords: ciona; motor ganglion; cholinergic locus; cholinergic ... See more keywords
Using Bacillus subtilis as a Host Cell to Express an Antimicrobial Peptide from the Marine Chordate Ciona intestinalis
Sign Up to like & getrecommendations! Published in 2021 at "Marine Drugs"
DOI: 10.3390/md19020111
Abstract: Ciona molecule against microbes-A24 (CiMAM) isolated from the marine chordate Ciona intestinalis is an antimicrobial peptide. To generate CiMAM-expressing transgenic Bacillus subtilis, we constructed a plasmid expressing recombinant CiMAM (rCiMAM) and introduced it into B.… read more here.
Keywords: ciona; marine; ciona intestinalis; antimicrobial peptide ... See more keywords