SpyCatcher-NTEV: A Circularly Permuted, Disordered SpyCatcher Variant for Less Trace Ligation.
Sign Up to like & getrecommendations! Published in 2018 at "Bioconjugate chemistry"
DOI: 10.1021/acs.bioconjchem.8b00131
Abstract: The SpyTag/SpyCatcher reaction has emerged as a powerful way for bioconjugation, but it leaves a folded complex in the product after the formation of the isopeptide bond. To vary the location of the reactive residue… read more here.
Keywords: circularly permuted; spycatcher ntev; reaction; trace ligation ... See more keywords
Circularly Permuted Fluorogenic Proteins for the Design of Modular Biosensors.
Sign Up to like & getrecommendations! Published in 2018 at "ACS chemical biology"
DOI: 10.1021/acschembio.8b00417
Abstract: Fluorescent reporters are essential components for the design of optical biosensors that are able to image intracellular analytes in living cells. Herein, we describe the development of circularly permuted variants of Fluorescence-Activating and absorption-Shifting Tag… read more here.
Keywords: design modular; fluorogenic proteins; permuted fluorogenic; biosensors circularly ... See more keywords
Circularly Permuted Fluorescent Protein-Based Indicators: History, Principles, and Classification
Sign Up to like & getrecommendations! Published in 2019 at "International Journal of Molecular Sciences"
DOI: 10.3390/ijms20174200
Abstract: Genetically encoded biosensors based on fluorescent proteins (FPs) are a reliable tool for studying the various biological processes in living systems. The circular permutation of single FPs led to the development of an extensive class… read more here.
Keywords: protein based; fluorescent protein; permuted fluorescent; based indicators ... See more keywords