One-step generation of zebrafish carrying a conditional knockout-knockin visible switch via CRISPR/Cas9-mediated intron targeting
Sign Up to like & getrecommendations! Published in 2019 at "Science China Life Sciences"
DOI: 10.1007/s11427-019-1607-9
Abstract: The zebrafish has become a popular vertebrate animal model in biomedical research. However, it is still challenging to make conditional gene knockout (CKO) models in zebrafish due to the low efficiency of homologous recombination (HR).… read more here.
Keywords: zckois; switch; one step; cko ... See more keywords
Oxidative stress resulting from the removal of endogenous catalase induces obesity by promoting hyperplasia and hypertrophy of white adipocytes
Sign Up to like & getrecommendations! Published in 2020 at "Redox Biology"
DOI: 10.1016/j.redox.2020.101749
Abstract: Obesity is regarded as an abnormal expansion and excessive accumulation of fat mass in white adipose tissue. The involvement of oxidative stress in the development of obesity is still unclear. Although mainly present in peroxisomes,… read more here.
Keywords: white adipocytes; cko; obesity; catalase ... See more keywords
Multi-omics analyses demonstrate a critical role for EHMT1 methyltransferase in transcriptional repression during oogenesis
Sign Up to like & getrecommendations! Published in 2022 at "Genome Research"
DOI: 10.1101/gr.277046.122
Abstract: GLP (EHMT1) is a multifunctional protein, best known for its role as an H3K9me1 and H3K9me2 methyltransferase through its reportedly obligatory dimerization with G9A (EHMT2). Here, we investigate the role of GLP in the oocyte… read more here.
Keywords: glp; g9a; glp cdko; cko ... See more keywords
Inducible deletion of endothelial cell EfnB2 attenuates neuromuscular regeneration in mouse skeletal muscle
Sign Up to like & getrecommendations! Published in 2023 at "Physiology"
DOI: 10.1152/physiol.2023.38.s1.5730060
Abstract: Background: Acute injury of skeletal muscle disrupts myofibers, microvessels and neuromuscular junctions (NMJs). While myofiber regeneration is well-characterized, little is known of concurrent events in microvessels and motor nerves following injury. Endothelial cell [EC] ephrin… read more here.
Keywords: muscle; physiology; cko; dpi ... See more keywords
Abstract 842: Protein kinase G - Regulator of G protein Signaling 2 Axis of Cardiac Myocytes Critically Determines Cardiac Performance in Early Cardiac Remodeling
Sign Up to like & getrecommendations! Published in 2019 at "Circulation Research"
DOI: 10.1161/res.125.suppl_1.842
Abstract: Introduction: Regulator of G protein signaling 2 (RGS2) is a negative regulator of G protein-coupled signaling, and is potentiated by cGMP-PKG. Our prior work using a global deletion model demonstrated the protective role for RGS2… read more here.
Keywords: protein; regulator protein; tac; cardiac remodeling ... See more keywords
Abstract 866: Cardiac-specific Deletion of General Control of Amino-Acid Synthesis 5-like 1 Regulates Fatty Acid Oxidation in Diet Induced Obesity
Sign Up to like & getrecommendations! Published in 2019 at "Circulation Research"
DOI: 10.1161/res.125.suppl_1.866
Abstract: Background: Healthy hearts use several fuel substrates for energy metabolism, with fatty acid oxidation (FAO) accounting for ~70% of total ATP production. Lack of flexibility to utilize different substrates, and a shift towards increased fatty… read more here.
Keywords: hfd; acetylation; fatty acid; fao ... See more keywords
m6A Maintains Hematopoietic Stem and Progenitor Cell Identity
Sign Up to like & getrecommendations! Published in 2018 at "Blood"
DOI: 10.1182/blood-2018-99-117191
Abstract: N-6-methyladenosine (m6A) is one of the most abundant posttranscriptional modifications in eukaryotic mRNAs and long noncoding RNAs. We previously found a critical role for m6A in promoting human myeloid leukemia (Vu et al. Nature Medicine… read more here.
Keywords: hscs; mettl3 cko; m6a; stem ... See more keywords
Steroidogenic factor 1 regulation of the hypothalamic-pituitary-ovarian axis of adult female mice.
Sign Up to like & getrecommendations! Published in 2022 at "Endocrinology"
DOI: 10.1210/endocr/bqac028
Abstract: The orphan nuclear receptor steroidogenic factor-1 (SF-1 or NR5A1) is an indispensable regulator of adrenal and gonadal formation, playing roles in sex determination, hypothalamic development, and pituitary function. This study aimed to identify the roles… read more here.
Keywords: cko; axis; hypothalamic pituitary; steroidogenic factor ... See more keywords
Uterine Dnmt3a is not required for mouse embryo implantation.
Sign Up to like & getrecommendations! Published in 2020 at "Current molecular medicine"
DOI: 10.2174/1566524020666200225115526
Abstract: BACKGROUND Recent studies have demonstrated that endometrial DNA methylation is essential for embryo implantation during early pregnancy. Dnmt3a is one of the key enzymes for DNA methylation and could be expressed in the endometrium regularly… read more here.
Keywords: uterine dnmt3a; embryo implantation; dnmt3a; cko ... See more keywords
Ubiquitin Carboxyl-Terminal Hydrolase L1 of Cardiomyocytes Promotes Macroautophagy and Proteostasis and Protects Against Post-myocardial Infarction Cardiac Remodeling and Heart Failure
Sign Up to like & getrecommendations! Published in 2022 at "Frontiers in Cardiovascular Medicine"
DOI: 10.3389/fcvm.2022.866901
Abstract: Ubiquitin carboxyl-terminal hydrolase L1 (UCHL1) is a deubiquitinase known to play essential roles in the nervous tissue. Myocardial upregulation of UCHL1 was observed in human dilated cardiomyopathy and several animal models of heart disease, but… read more here.
Keywords: mice; terminal hydrolase; heart; ubiquitin carboxyl ... See more keywords
Neuron-Specific Deletion of Scrib in Mice Leads to Neuroanatomical and Locomotor Deficits
Sign Up to like & getrecommendations! Published in 2022 at "Frontiers in Genetics"
DOI: 10.3389/fgene.2022.872700
Abstract: Scribble (Scrib) is a conserved polarity protein acting as a scaffold involved in multiple cellular and developmental processes. Recent evidence from our group indicates that Scrib is also essential for brain development as early global… read more here.
Keywords: deletion scrib; scrib cko; scrib; cko ... See more keywords