Unexpected new insights into DNA clamp loaders
Sign Up to like & getrecommendations! Published in 2022 at "Bioessays"
DOI: 10.1002/bies.202200154
Abstract: Clamp loaders are pentameric AAA+ assemblies that use ATP to open and close circular DNA sliding clamps around DNA. Clamp loaders show homology in all organisms, from bacteria to human. The eukaryotic PCNA clamp is… read more here.
Keywords: clamp loaders; clamp; dna; dna clamp ... See more keywords
Nucleated, Outside-Out, Somatic, Macropatch Recordings in Native Neurons.
Sign Up to like & getrecommendations! Published in 2021 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-0818-0_11
Abstract: Patch-clamp recordings are a powerful tool for the live measurement of the plasma membrane biophysical properties, with the ability to discriminate fast events such as fast inactivating Na+ currents ( read more here.
Keywords: clamp recordings; patch clamp; native neurons; clamp ... See more keywords
Patch Clamp Technology in the Twenty-First Century.
Sign Up to like & getrecommendations! Published in 2021 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-0818-0_2
Abstract: In the almost four decades since its inception, the patch clamp technique has transitioned from a specialist skill to a method commonly used among many others in a lab. Development of patch clamp instrumentation has… read more here.
Keywords: clamp technology; technology twenty; twenty first; patch clamp ... See more keywords
Posterolateral clamp placement on the pelvic brim provides optimal reduction of transtectal acetabular fractures fixed through the anterior intrapelvic approach
Sign Up to like & getrecommendations! Published in 2019 at "International Orthopaedics"
DOI: 10.1007/s00264-019-04430-x
Abstract: Purpose To determine the optimal position of reduction clamps applied to reduce transtectal transverse acetabular fractures approached through the anterior intrapelvic route. Methods Transtectal transverse fractures were created in sawbone hemipelvis models. Fractures were reduced… read more here.
Keywords: reduction; pelvic brim; clamp; approach ... See more keywords
Off-clamp robot-assisted partial nephrectomy does not benefit short-term renal function: a matched cohort analysis
Sign Up to like & getrecommendations! Published in 2017 at "Journal of Robotic Surgery"
DOI: 10.1007/s11701-017-0745-6
Abstract: In the interest of renal functional preservation, partial nephrectomy has supplanted radical nephrectomy as the preferred treatment for T1 renal masses. This procedure usually involves the induction of renal warm ischemia by clamping the hilar… read more here.
Keywords: partial nephrectomy; renal functional; clamp rapn; cohort ... See more keywords
Advances in the automation of whole-cell patch clamp technology
Sign Up to like & getrecommendations! Published in 2019 at "Journal of Neuroscience Methods"
DOI: 10.1016/j.jneumeth.2019.108357
Abstract: Electrophysiology is the study of neural activity in the form of local field potentials, current flow through ion channels, calcium spikes, back propagating action potentials and somatic action potentials, all measurable on a millisecond timescale.… read more here.
Keywords: patch clamp; clamp; clamp technology; action potentials ... See more keywords
Dual Roles of Ctf18-RFC: Loading the Clamp and Angling for the Polymerase.
Sign Up to like & getrecommendations! Published in 2018 at "Structure"
DOI: 10.1016/j.str.2017.12.009
Abstract: Clamp loaders such as Ctf18-RFC assist DNA replication by loading the PCNA clamp onto DNA. In this issue of Structure, Grabarczyk et al. (2018) reveal that a hook-like structure formed by the unique Dcc1 and Ctf8… read more here.
Keywords: rfc loading; roles ctf18; clamp; ctf18 rfc ... See more keywords
Comparing Off-clamp and On-clamp Robot-assisted Partial Nephrectomy: A Prospective Randomized Trial.
Sign Up to like & getrecommendations! Published in 2019 at "Urology"
DOI: 10.1016/j.urology.2018.11.053
Abstract: OBJECTIVE To determine whether performing robot-assisted partial nephrectomy without warm ischemia "off-clamp" results in favorable postoperative renal functional outcomes compared with the on-clamp method. METHODS We conducted a prospective trial of 80 patients who underwent… read more here.
Keywords: partial nephrectomy; clamp clamp; assisted partial; robot assisted ... See more keywords
An “All Inclusive “Package for Cardiac Safety: The Six Big on One Automated Patch Clamp Chip
Sign Up to like & getrecommendations! Published in 2017 at "Journal of Pharmacological and Toxicological Methods"
DOI: 10.1016/j.vascn.2017.09.090
Abstract: Drug discovery is challenged by poor predictability of proarrhythmic effects of present cardiac safety screening approaches. The Comprehensive in Vitro Proarrhythmia Assay (CiPA), an FDA directed initiative, is specifically focused on improved preclinical assessment of… read more here.
Keywords: cardiac safety; package cardiac; inclusive package; safety ... See more keywords
Structural basis of human PCNA sliding on DNA
Sign Up to like & getrecommendations! Published in 2017 at "Nature Communications"
DOI: 10.1038/ncomms13935
Abstract: Sliding clamps encircle DNA and tether polymerases and other factors to the genomic template. However, the molecular mechanism of clamp sliding on DNA is unknown. Using crystallography, NMR and molecular dynamics simulations, here we show… read more here.
Keywords: pcna; structural basis; dna; clamp ... See more keywords
Hypoxia and exercise interactions on skeletal muscle insulin sensitivity in obese subjects with metabolic syndrome: results of a randomized controlled trial
Sign Up to like & getrecommendations! Published in 2019 at "International Journal of Obesity"
DOI: 10.1038/s41366-019-0504-z
Abstract: Background Physical activity improves insulin sensitivity in obesity. Hypoxia training is claimed to augment this effect. We tested the hypothesis that normobaric hypoxia training would improve insulin sensitivity in obese patients with metabolic syndrome. Methods… read more here.
Keywords: training; sensitivity; clamp; metabolic syndrome ... See more keywords