Unexpected new insights into DNA clamp loaders
Sign Up to like & getrecommendations! Published in 2022 at "Bioessays"
DOI: 10.1002/bies.202200154
Abstract: Clamp loaders are pentameric AAA+ assemblies that use ATP to open and close circular DNA sliding clamps around DNA. Clamp loaders show homology in all organisms, from bacteria to human. The eukaryotic PCNA clamp is… read more here.
Keywords: clamp loaders; clamp; dna; dna clamp ... See more keywords
The Escherichia coli clamp loader rapidly remodels SSB on DNA to load clamps
Sign Up to like & getrecommendations! Published in 2022 at "Nucleic Acids Research"
DOI: 10.1093/nar/gkac1169
Abstract: Abstract Single-stranded DNA binding proteins (SSBs) avidly bind ssDNA and yet enzymes that need to act during DNA replication and repair are not generally impeded by SSB, and are often stimulated by SSB. Here, the… read more here.
Keywords: clamp loader; dna; ssb dna;
Mechanism of opening a sliding clamp
Sign Up to like & getrecommendations! Published in 2017 at "Nucleic Acids Research"
DOI: 10.1093/nar/gkx665
Abstract: Abstract Clamp loaders load ring-shaped sliding clamps onto DNA where the clamps serve as processivity factors for DNA polymerases. In the first stage of clamp loading, clamp loaders bind and stabilize clamps in an open… read more here.
Keywords: clamp loader; sliding clamp; mechanism; clamp loaders ... See more keywords
Structure of the human RAD17–RFC clamp loader and 9–1–1 checkpoint clamp bound to a dsDNA–ssDNA junction
Sign Up to like & getrecommendations! Published in 2022 at "Nucleic Acids Research"
DOI: 10.1101/2022.03.11.484023
Abstract: The RAD9-RAD1-HUS1 (9-1-1) clamp forms one half of the DNA damage checkpoint system that signals the presence of substantial regions of single-stranded DNA arising from replication fork collapse or resection of DNA double strand breaks.… read more here.
Keywords: rfc clamp; clamp; dsdna ssdna; rad17 rfc ... See more keywords
Distinct Functions in Regulation of Meiotic Crossovers for DNA Damage Response Clamp Loader Rad24(Rad17) and Mec1(ATR) Kinase.
Sign Up to like & getrecommendations! Published in 2019 at "Genetics"
DOI: 10.1534/genetics.119.302427
Abstract: The number and distribution of meiotic crossovers (COs) are highly regulated, reflecting the requirement for COs during the first round of meiotic chromosome segregation. CO control includes CO assurance and CO interference, which promote at… read more here.
Keywords: rad24; clamp loader; mec1; clamp ... See more keywords
Cryo-EM structures reveal high-resolution mechanism of a DNA polymerase sliding clamp loader
Sign Up to like & getrecommendations! Published in 2022 at "eLife"
DOI: 10.7554/elife.74175
Abstract: Sliding clamps are ring-shaped protein complexes that are integral to the DNA replication machinery of all life. Sliding clamps are opened and installed onto DNA by clamp loader AAA+ ATPase complexes. However, how a clamp… read more here.
Keywords: rfc; clamp loader; sliding clamp; dna ... See more keywords