SnapShot: Class 2 CRISPR-Cas Systems
Sign Up to like & getrecommendations! Published in 2017 at "Cell"
DOI: 10.1016/j.cell.2016.12.038
Abstract: Class 2 CRISPR-Cas systems are characterized by effector modules consisting of single, large, multidomain proteins that appear to have been derived from mobile genetic elements. Some Class 2 effector proteins, such as Cas9 and Cas12a… read more here.
Keywords: class; crispr cas; cas systems; class crispr ... See more keywords
Building the Class 2 CRISPR-Cas Arsenal.
Sign Up to like & getrecommendations! Published in 2017 at "Molecular cell"
DOI: 10.1016/j.molcel.2017.01.024
Abstract: Adaptation of CRISPR-Cas9 for genome-editing applications has revolutionized biomedical research. New single-component effector CRISPR systems are emerging from the bioinformatics pipeline. How can we best harness their power? Three new studies will no doubt facilitate… read more here.
Keywords: building class; cas arsenal; crispr; crispr cas ... See more keywords
Mechanistic insights into the versatile class II CRISPR toolbox.
Sign Up to like & getrecommendations! Published in 2021 at "Trends in biochemical sciences"
DOI: 10.1016/j.tibs.2021.11.007
Abstract: The constantly expanding group of class II CRISPR-Cas (clustered regularly interspaced short palindromic repeats-associated) effectors and their engineered variants exhibit distinct editing modes and efficiency, fidelity, target range, and molecular size. Their enormous diversity of… read more here.
Keywords: versatile class; class; mechanistic insights; insights versatile ... See more keywords
Comparative genomics and evolution of trans-activating RNAs in Class 2 CRISPR-Cas systems
Sign Up to like & getrecommendations! Published in 2019 at "RNA Biology"
DOI: 10.1080/15476286.2018.1493331
Abstract: ABSTRACT Trans-activating CRISPR (tracr) RNA is a distinct RNA species that interacts with the CRISPR (cr) RNA to form the dual guide (g) RNA in type II and subtype V-B CRISPR-Cas systems. The tracrRNA-crRNA interaction… read more here.
Keywords: crispr; crispr cas; class crispr; cas systems ... See more keywords
Predicted highly derived class 1 CRISPR-Cas system in Haloarchaea containing diverged Cas5 and Cas7 homologs but no CRISPR array.
Sign Up to like & getrecommendations! Published in 2019 at "FEMS microbiology letters"
DOI: 10.1093/femsle/fnz079
Abstract: Screening of genomic and metagenomic databases for new variants of CRISPR-Cas systems increasingly results in the discovery of derived variants that do not seem to possess the interference capacity and are implicated in functions distinct… read more here.
Keywords: crispr cas; class crispr; cas system; crispr ... See more keywords