Purification of Clathrin-Coated Vesicles from Adult Rat Brain.
Sign Up to like & getrecommendations! Published in 2018 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-8719-1_1
Abstract: Here, we describe a purification protocol for isolating clathrin-coated vesicles (CCVs) from adult rat brain by using differential centrifugation coupled with Ficoll-sucrose and D2O-sucrose density gradient centrifugation and an additional linear sucrose step gradient at… read more here.
Keywords: adult rat; purification; clathrin coated; rat brain ... See more keywords
Temporal Ordering in Endocytic Clathrin-Coated Vesicle Formation via AP2 Phosphorylation
Sign Up to like & getrecommendations! Published in 2019 at "Developmental Cell"
DOI: 10.1016/j.devcel.2019.07.017
Abstract: Summary Clathrin-mediated endocytosis (CME) is key to maintaining the transmembrane protein composition of cells’ limiting membranes. During mammalian CME, a reversible phosphorylation event occurs on Thr156 of the μ2 subunit of the main endocytic clathrin… read more here.
Keywords: phosphorylation; endocytic clathrin; formation; clathrin coated ... See more keywords
A new role of anterograde motor Kif5b in facilitating large clathrin-coated vesicle mediated endocytosis via regulating clathrin uncoating
Sign Up to like & getrecommendations! Published in 2018 at "Cell Discovery"
DOI: 10.1038/s41421-018-0067-5
Abstract: Kif5b-driven anterograde transport and clathrin-mediated endocytosis (CME) are responsible for opposite intracellular trafficking, contributing to plasma membrane homeostasis. However, whether and how the two trafficking processes coordinate remain unclear. Here, we show that Kif5b directly… read more here.
Keywords: new role; large clathrin; clathrin coated; mediated endocytosis ... See more keywords
REEP6 mediates trafficking of a subset of Clathrin-coated vesicles and is critical for rod photoreceptor function and survival
Sign Up to like & getrecommendations! Published in 2017 at "Human Molecular Genetics"
DOI: 10.1093/hmg/ddx111
Abstract: Abstract In retinal photoreceptors, vectorial transport of cargo is critical for transduction of visual signals, and defects in intracellular trafficking can lead to photoreceptor degeneration and vision impairment. Molecular signatures associated with routing of transport… read more here.
Keywords: rod photoreceptor; reep6; clathrin coated; rod ... See more keywords
Architecture of the AP2/clathrin coat on the membranes of clathrin-coated vesicles
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DOI: 10.1126/sciadv.aba8381
Abstract: Cryo-electron tomography reveals the conformational changes and clathrin interactions of AP2 during coated vesicle assembly. Clathrin-mediated endocytosis (CME) is crucial for modulating the protein composition of a cell’s plasma membrane. Clathrin forms a cage-like, polyhedral… read more here.
Keywords: architecture ap2; clathrin coated; coat; ap2 clathrin ... See more keywords
The structures of natively assembled clathrin-coated vesicles
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DOI: 10.1126/sciadv.aba8397
Abstract: Distribution of adaptor proteins in clathrin-coated vesicles is not random. Clathrin-coated vesicles mediate trafficking of proteins and nutrients in the cell and between organelles. Proteins included in the clathrin-coated vesicles (CCVs) category include clathrin heavy… read more here.
Keywords: hexagonal faces; clathrin coated; structures natively; coated vesicles ... See more keywords
Helping a cell to migrate in 3D space.
Sign Up to like & getrecommendations! Published in 2017 at "Science"
DOI: 10.1126/science.356.6343.1134-m
Abstract: Cell Adhesion Clathrin-coated pits are well known to be involved in receptor-mediated endocytosis. Independent of their role in endocytosis, Elkhatib et al. observed that clathrin-coated structures strongly accumulated along collagen fibers in migrating cells. Clathrin-coated… read more here.
Keywords: cell migrate; migrate space; helping cell; clathrin coated ... See more keywords
Loss of PTEN promotes formation of signaling-capable clathrin-coated pits
Sign Up to like & getrecommendations! Published in 2018 at "Journal of Cell Science"
DOI: 10.1242/jcs.208926
Abstract: ABSTRACT Defective endocytosis and vesicular trafficking of signaling receptors has recently emerged as a multifaceted hallmark of malignant cells. Clathrin-coated pits (CCPs) display highly heterogeneous dynamics on the plasma membrane where they can take from… read more here.
Keywords: coated pits; clathrin coated; loss pten; signaling capable ... See more keywords
Endocytosis at extremes: Formation and internalization of giant clathrin-coated pits under elevated membrane tension
Sign Up to like & getrecommendations! Published in 2022 at "Frontiers in Molecular Biosciences"
DOI: 10.3389/fmolb.2022.959737
Abstract: Internalization of clathrin-coated vesicles from the plasma membrane constitutes the major endocytic route for receptors and their ligands. Dynamic and structural properties of endocytic clathrin coats are regulated by the mechanical properties of the plasma… read more here.
Keywords: coated pits; internalization; membrane tension; clathrin ... See more keywords
Preparation of Clathrin-Coated Vesicles From Arabidopsis thaliana Seedlings
Sign Up to like & getrecommendations! Published in 2019 at "Frontiers in Plant Science"
DOI: 10.3389/fpls.2018.01972
Abstract: Clathrin coated vesicles (CCVs) mediate endocytosis of plasma membrane proteins and deliver their content to the endosomes for either subsequent recycling to the plasma membrane or transport to the vacuole for degradation. CCVs assemble also… read more here.
Keywords: ccvs; thaliana seedlings; arabidopsis thaliana; clathrin coated ... See more keywords