CRISPR-Trap: a clean approach for the generation of gene knockouts and gene replacements in human cells
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DOI: 10.1091/mbc.e17-05-0288
Abstract: Conventional nonhomologous end joining–based gene knockouts can lead to the production of C-terminally truncated proteins with potentially residual or dominant negative functions. Combining CRISPR/Cas9 with gene traps targeting the first intron (CRISPR-Trap), however, completely prevents… read more here.
Keywords: gene knockouts; approach generation; clean approach; trap clean ... See more keywords