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   Articles with "cleavage activity" as a keyword



Unveiling Cas12j Trans‐Cleavage Activity for CRISPR Diagnostics: Application to miRNA Detection in Lung Cancer Diagnosis

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recommendations! Published in 2024 at "Advanced Science"

DOI: 10.1002/advs.202402580

Abstract: Cas12j, a hypercompact and efficient Cas protein, has potential for use in CRISPR diagnostics, but has not yet been used because the trans‐cleavage activity of Cas12j is veiled. Here, the trans‐cleavage behavior of Cas12j1, 2,… read more here.

Keywords: detection; crispr diagnostics; trans cleavage; cleavage activity ... See more keywords

Developing a capillary electrophoresis based method for dynamically monitoring enzyme cleavage activity using quantum dots‐peptide assembly

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recommendations! Published in 2017 at "ELECTROPHORESIS"

DOI: 10.1002/elps.201700117

Abstract: Herein, a novel assay has been developed for monitoring PreScission protease (His‐PSP) mediated enzyme cleavage of ATTO 590 labeled peptide substrate (ATTO‐LEV). This novel method is based on combining the use of capillary electrophoresis and… read more here.

Keywords: enzyme cleavage; capillary electrophoresis; cleavage; method ... See more keywords

"RESET" Effect: Random Extending Sequences Enhance the Trans-Cleavage Activity of CRISPR/Cas12a.

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recommendations! Published in 2022 at "Analytical chemistry"

DOI: 10.1021/acs.analchem.2c01401

Abstract: The trans-cleavage activity of CRISPR/Cas12a has been widely used in biosensing applications. However, the lack of exploration on the fundamental properties of CRISPR/Cas12a not only discourages further in-depth studies of the CRISPR/Cas12a system but also… read more here.

Keywords: crispr cas12a; trans cleavage; cas12a; cleavage activity ... See more keywords

Signal Amplification by the trans-Cleavage Activity of CRISPR-Cas Systems: Kinetics and Performance

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recommendations! Published in 2023 at "Analytical Chemistry"

DOI: 10.1021/acs.analchem.2c04555

Abstract: ACCESS Metrics & More Article Recommendations ■ CONTENTS Functional Basics of Cas12 and Cas13 208 Nuclease Activity of Cas12a 208 Nuclease Activity of Cas13a 208 Quantitative Determination of trans-Cleavage Kinetics of Cas Enzymes 209 trans-Cleavage… read more here.

Keywords: signal amplification; trans cleavage; cleavage kinetics; activity ... See more keywords

Exploring the Effect of Steric Hindrance on Trans-cleavage Activity of CRISPR-cas12a for Ultrasensitive SERS Detection of P53 DNA.

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recommendations! Published in 2024 at "Analytical chemistry"

DOI: 10.1021/acs.analchem.4c01347

Abstract: The trans-cleavage properties of Cas12a make it important for gene editing and disease diagnosis. In this work, the effect of spatial site resistance on the trans-cleavage activity of Cas12a was studied. First, we have explored… read more here.

Keywords: trans cleavage; cleavage activity; effect; cas12a ... See more keywords

Real-Time FO-SPR Monitoring of Solid-Phase DNAzyme Cleavage Activity for Cutting-Edge Biosensing.

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recommendations! Published in 2019 at "ACS applied materials & interfaces"

DOI: 10.1021/acsami.8b18756

Abstract: DNA nanotechnology has a great potential in biosensor design including nanostructuring of the biosensor surface through DNA origami, target recognition by means of aptamers, and DNA-based signal amplification strategies. In this paper, we use DNA… read more here.

Keywords: cleavage; time; target; cleavage activity ... See more keywords

Structural insights into Type II-D Cas9 and its robust cleavage activity

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recommendations! Published in 2025 at "Nature Communications"

DOI: 10.1038/s41467-025-62128-8

Abstract: Type II-D Cas9 proteins (Cas9d) are more compact than typical Type II-A/B/C Cas9s. Here, we demonstrate that NsCas9d from Nitrospirae bacterium RBG_13_39_12 derived from a metagenomic assembly exhibits robust dsDNA cleavage activity comparable to SpCas9… read more here.

Keywords: type cas9; cas9; cleavage activity; cleavage ... See more keywords

CRISPR/Cas12a collateral cleavage activity for an ultrasensitive assay of RNase H.

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recommendations! Published in 2022 at "Chemical communications"

DOI: 10.1039/d1cc06026k

Abstract: We herein describe an ultrasensitive RNase H assay by utilizing CRISPR/Cas12a collateral cleavage activity. Based on this unique design principle, the RNase H activity was successfully determined down to 0.00024 U mL-1, which is quite… read more here.

Keywords: cas12a collateral; rnase; activity; collateral cleavage ... See more keywords

Influence of sequence variation on the RNA cleavage activity of Zn2+-dimethyl-dppz-PNA-based artificial enzymes

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recommendations! Published in 2022 at "RSC Advances"

DOI: 10.1039/d1ra08319h

Abstract: The development of Zn2+-dependent dimethyl-dppz-PNA conjugates (PNAzymes) as efficient site-specific artificial ribonucleases enables rapid sequence-specific degradation of clinically relevant RNA target sequences, but the significance of the RNA/PNAzyme sequence and structural demands for the identification… read more here.

Keywords: rna cleavage; cleavage; sequence; cleavage activity ... See more keywords

Loosely-packed dynamical structures with partially-melted surface being the key for thermophilic argonaute proteins achieving high DNA-cleavage activity

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recommendations! Published in 2022 at "Nucleic Acids Research"

DOI: 10.1093/nar/gkac565

Abstract: Abstract Prokaryotic Argonaute proteins (pAgos) widely participate in hosts to defend against the invasion of nucleic acids. Compared with the CRISPR-Cas system, which requires a specific motif on the target and can only use RNA… read more here.

Keywords: dna; structure; thermophilic pagos; activity ... See more keywords

Enhancement of CRISPR/Cas12a trans-cleavage activity using hairpin DNA reporters

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recommendations! Published in 2022 at "Nucleic Acids Research"

DOI: 10.1093/nar/gkac578

Abstract: Abstract The RNA programmed non-specific (trans) nuclease activity of CRISPR-Cas Type V and VI systems has opened a new era in the field of nucleic acid-based detection. Here, we report on the enhancement of trans-cleavage… read more here.

Keywords: hairpin dna; dna; trans cleavage; cleavage activity ... See more keywords