Developing a capillary electrophoresis based method for dynamically monitoring enzyme cleavage activity using quantum dots‐peptide assembly
Sign Up to like & getrecommendations! Published in 2017 at "ELECTROPHORESIS"
DOI: 10.1002/elps.201700117
Abstract: Herein, a novel assay has been developed for monitoring PreScission protease (His‐PSP) mediated enzyme cleavage of ATTO 590 labeled peptide substrate (ATTO‐LEV). This novel method is based on combining the use of capillary electrophoresis and… read more here.
Keywords: enzyme cleavage; capillary electrophoresis; cleavage; method ... See more keywords
"RESET" Effect: Random Extending Sequences Enhance the Trans-Cleavage Activity of CRISPR/Cas12a.
Sign Up to like & getrecommendations! Published in 2022 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.2c01401
Abstract: The trans-cleavage activity of CRISPR/Cas12a has been widely used in biosensing applications. However, the lack of exploration on the fundamental properties of CRISPR/Cas12a not only discourages further in-depth studies of the CRISPR/Cas12a system but also… read more here.
Keywords: crispr cas12a; trans cleavage; cas12a; cleavage activity ... See more keywords
Signal Amplification by the trans-Cleavage Activity of CRISPR-Cas Systems: Kinetics and Performance
Sign Up to like & getrecommendations! Published in 2023 at "Analytical Chemistry"
DOI: 10.1021/acs.analchem.2c04555
Abstract: ACCESS Metrics & More Article Recommendations ■ CONTENTS Functional Basics of Cas12 and Cas13 208 Nuclease Activity of Cas12a 208 Nuclease Activity of Cas13a 208 Quantitative Determination of trans-Cleavage Kinetics of Cas Enzymes 209 trans-Cleavage… read more here.
Keywords: signal amplification; trans cleavage; cleavage kinetics; activity ... See more keywords
Real-Time FO-SPR Monitoring of Solid-Phase DNAzyme Cleavage Activity for Cutting-Edge Biosensing.
Sign Up to like & getrecommendations! Published in 2019 at "ACS applied materials & interfaces"
DOI: 10.1021/acsami.8b18756
Abstract: DNA nanotechnology has a great potential in biosensor design including nanostructuring of the biosensor surface through DNA origami, target recognition by means of aptamers, and DNA-based signal amplification strategies. In this paper, we use DNA… read more here.
Keywords: cleavage; time; target; cleavage activity ... See more keywords
CRISPR/Cas12a collateral cleavage activity for an ultrasensitive assay of RNase H.
Sign Up to like & getrecommendations! Published in 2022 at "Chemical communications"
DOI: 10.1039/d1cc06026k
Abstract: We herein describe an ultrasensitive RNase H assay by utilizing CRISPR/Cas12a collateral cleavage activity. Based on this unique design principle, the RNase H activity was successfully determined down to 0.00024 U mL-1, which is quite… read more here.
Keywords: cas12a collateral; rnase; activity; collateral cleavage ... See more keywords
Influence of sequence variation on the RNA cleavage activity of Zn2+-dimethyl-dppz-PNA-based artificial enzymes
Sign Up to like & getrecommendations! Published in 2022 at "RSC Advances"
DOI: 10.1039/d1ra08319h
Abstract: The development of Zn2+-dependent dimethyl-dppz-PNA conjugates (PNAzymes) as efficient site-specific artificial ribonucleases enables rapid sequence-specific degradation of clinically relevant RNA target sequences, but the significance of the RNA/PNAzyme sequence and structural demands for the identification… read more here.
Keywords: rna cleavage; cleavage; sequence; cleavage activity ... See more keywords
Loosely-packed dynamical structures with partially-melted surface being the key for thermophilic argonaute proteins achieving high DNA-cleavage activity
Sign Up to like & getrecommendations! Published in 2022 at "Nucleic Acids Research"
DOI: 10.1093/nar/gkac565
Abstract: Abstract Prokaryotic Argonaute proteins (pAgos) widely participate in hosts to defend against the invasion of nucleic acids. Compared with the CRISPR-Cas system, which requires a specific motif on the target and can only use RNA… read more here.
Keywords: dna; structure; thermophilic pagos; activity ... See more keywords
Enhancement of CRISPR/Cas12a trans-cleavage activity using hairpin DNA reporters
Sign Up to like & getrecommendations! Published in 2022 at "Nucleic Acids Research"
DOI: 10.1093/nar/gkac578
Abstract: Abstract The RNA programmed non-specific (trans) nuclease activity of CRISPR-Cas Type V and VI systems has opened a new era in the field of nucleic acid-based detection. Here, we report on the enhancement of trans-cleavage… read more here.
Keywords: hairpin dna; dna; trans cleavage; cleavage activity ... See more keywords