The binacle (binding and cleavage) assay for in vitro determination of botulinum neurotoxin activity
Sign Up to like & getrecommendations! Published in 2018 at "Toxicon"
DOI: 10.1016/j.toxicon.2018.11.026
Abstract: Principle of the BINACLE assay. (I.) BoNT molecules (grey) bind to immobilized receptors (blue and red) via their H-chains. Then the L-chains are released by reduction of the disulfide bonds. (II.) The supernatant with the… read more here.
Keywords: binding cleavage; cleavage; binacle binding; binacle ... See more keywords
Heteroduplex cleavage assay for screening of probable zygosities resulting from CRISPR mutations in diploid single cell lines.
Sign Up to like & getrecommendations! Published in 2017 at "BioTechniques"
DOI: 10.2144/000114556
Abstract: The most common gene editing methods, such as CRISPR, involve random repair of an induced double-stranded DNA break through the non-homologous end joining (NHEJ) repair pathway, resulting in small insertions/deletions. In diploid cells, these mutations… read more here.
Keywords: gene editing; dna sequencing; heteroduplex cleavage; gene ... See more keywords
Generation of Matrix Degradation Products Using an In Vitro MMP Cleavage Assay
Sign Up to like & getrecommendations! Published in 2022 at "International Journal of Molecular Sciences"
DOI: 10.3390/ijms23116245
Abstract: Matrix metalloproteinases (MMPs) play crucial roles in tissue homeostasis and pathologies by remodeling the extracellular matrix. Previous studies have demonstrated the biological activities of MMP-derived cleavage products. Furthermore, specific fragments can serve as biomarkers. Therefore,… read more here.
Keywords: vitro cleavage; cleavage; matrix degradation; generation matrix ... See more keywords