Development of Quenching-qPCR (Q-Q) assay for measuring absolute intracellular cleavage efficiency of ribozyme.
Sign Up to like & getrecommendations! Published in 2018 at "Analytical biochemistry"
DOI: 10.1016/j.ab.2018.04.007
Abstract: Ribozyme (Rz) is a very attractive RNA molecule in metabolic engineering and synthetic biology fields where RNA processing is required as a control unit or ON/OFF signal for its cleavage reaction. In order to use… read more here.
Keywords: cleavage efficiency; cleavage; ribozyme; intracellular cleavage ... See more keywords
CRISPR/Cas9 cleavage efficiency regression through boosting algorithms and Markov sequence profiling
Sign Up to like & getrecommendations! Published in 2018 at "Bioinformatics"
DOI: 10.1093/bioinformatics/bty298
Abstract: Motivation CRISPR/Cas9 system is a widely used genome editing tool. A prediction problem of great interests for this system is: how to select optimal single-guide RNAs (sgRNAs), such that its cleavage efficiency is high meanwhile… read more here.
Keywords: markov; regression; cleavage efficiency; cleavage ... See more keywords